Difference between revisions of "Oroboros FAQ"
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== O2k FAQ == | === O2k FAQ === | ||
== APPLICATIONS == | |||
<span style="color:#FFF8F5">blue writing</span> | |||
<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Is the OROBOROS Oxygraph-2k (O2k) easy to use in standard applications of mitochondrial respiration?</div> | |||
The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: [[DatLab]] | |||
<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?</div> | |||
'' | |||
The O2k can be routinely applied for cultured cells, intact cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: [[Respirometry]] | The O2k can be routinely applied for cultured cells, intact cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: [[Respirometry]] | ||
<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">What is special about high-resolution respirometry?</div> | |||
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. | The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. | ||
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<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Which are the most common applications of the O2k?</div> | |||
The most common applications for the OROBOROS O2k are the following: | ''The most common applications for the OROBOROS O2k are the following'': | ||
* Diagnosis of acquired and genetic mitochondrial diseases | * Diagnosis of acquired and genetic mitochondrial diseases | ||
* Diagnosis of myopathies and neuromuscular pathologies | * Diagnosis of myopathies and neuromuscular pathologies | ||
* Studies on cell function and cell death - apoptosis and necrosis | * Studies on cell function and cell death - apoptosis and necrosis | ||
* | * Measurement of oxidative stress | ||
* | * Studies on ischemia-reperfusion injury | ||
* | * Studies on aging and senescence | ||
* Pharmacological tests | * Pharmacological tests | ||
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<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?</div> | |||
Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personell- and time-efficient. | |||
<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Can the O2k be applied for measuring human biopsies?</div> | |||
When permeabilized biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols. | |||
<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Can the O2k be applied to measure tissue oxygen concentration?</div> | |||
The O2k is a 'chamber' approach, for application with tissue biopsies, cultured cells or isolated mitochondria. Whereas HRR cannot be applied for measurement of tissue | The O2k is a 'chamber' approach, for application with tissue biopsies, cultured cells or isolated mitochondria. Whereas HRR cannot be applied for measurement of tissue O<sup>2</sup> concentration, it is the method of choice for analysis of tissue O<sup>2</sup> consumption and functional diagnosis of mitochondrial injuries. | ||
<div style="border-bottom:1px solid #abd5f5; background:#d0e5f5; font-size:110%; font-weight:bold;">Can the Oxygraph-2k be applied for the measurement of oxygen consumption in adherent cells?</div> | |||
Attached cells may be grown on disks before being introduced into the cylindrical chamber of the O2k. Alternatively, cells attached to beads can be used. With this method, however, gentle stirring, with the use of specific small stirrer flees, is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, 200,000 cells per chamber are required. | |||
* How many parameters can be measured simultaneously? | |||
(respiration + 1 additional parameter) | |||
== HOW AND WHERE TO BUY == | |||
<div style="border-bottom:1px solid #abd5f5; background:#a1a1a2; font-size:110%; font-weight:bold;">Do you have any distributors in my country?</div> | |||
(exclusive distributors in XXX, direct sales approach, if necessary cooperate with a distributor you choose) | |||
== DELIVERY CONTENT, WARRANTY AND SUPPORT == | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Does the purchase include installation and setup and training?</div> | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Do you have an after-sales service contract?</div> | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Does the O2k-Core include a PC or do we have to purchase it separately?</div> | |||
Verlinkung zu PC requirements und dann auch electrical requirements | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Is it possible to extend the warranty period?</div> | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Are taxes and duty fees included in the price?</div> | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Do you offer the option to lease or rent your instruments?</div> | |||
<div style="border-bottom:1px solid #abd5f5; background:#c0f5a5; font-size:110%; font-weight:bold;">Are you registered with System for Award Management (SAM)?</div> | |||
Revision as of 13:05, 21 December 2015
O2k FAQ
APPLICATIONS
blue writing
The O2k is a robust instrument, ready to be used for standard applications, providing high reliability, stability of the oxygen sensor over several months, and full software support. See: DatLab
The O2k can be routinely applied for cultured cells, intact cells and tissue biopsies, permeabilized cells and tissue biopsies, microbial cultures, oxygen-consuming enzymes, antioxidant systems, and, of course, isolated mitochondria. See: Respirometry
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry.
O2k high-resolution respirometry (HRR) is based on the OROBOROS O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation (TIP2k). See: High-Resolution_Respirometry
The most common applications for the OROBOROS O2k are the following:
- Diagnosis of acquired and genetic mitochondrial diseases
- Diagnosis of myopathies and neuromuscular pathologies
- Studies on cell function and cell death - apoptosis and necrosis
- Measurement of oxidative stress
- Studies on ischemia-reperfusion injury
- Studies on aging and senescence
- Pharmacological tests
For more applications, browse through the O2k-Publications.
Compared to other instruments, the O2k requires five to ten times lower cell numbers for routine measurements of cell respiration, making it highly cost-, personell- and time-efficient.
When permeabilized biopsy preparations are utilized, the O2k provides the unique potential to reduce sample size to as little as 1 mg fresh weight per experimental run, supporting application of complex titration protocols.
The O2k is a 'chamber' approach, for application with tissue biopsies, cultured cells or isolated mitochondria. Whereas HRR cannot be applied for measurement of tissue O2 concentration, it is the method of choice for analysis of tissue O2 consumption and functional diagnosis of mitochondrial injuries.
Attached cells may be grown on disks before being introduced into the cylindrical chamber of the O2k. Alternatively, cells attached to beads can be used. With this method, however, gentle stirring, with the use of specific small stirrer flees, is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, 200,000 cells per chamber are required.
- How many parameters can be measured simultaneously?
(respiration + 1 additional parameter)
HOW AND WHERE TO BUY
(exclusive distributors in XXX, direct sales approach, if necessary cooperate with a distributor you choose)
DELIVERY CONTENT, WARRANTY AND SUPPORT
Verlinkung zu PC requirements und dann auch electrical requirements
