Open chamber
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The term "open chamber" is used to denote a situation in which the liquid phase is in equilibrium with the atmosphere, resulting in a net zero O2 flux at open chamber. To maintain signal stability and protection from contamination this is NOT done by leaving the chamber open (no stoppers) but by creating a defined gas phase in the chamber between liquid phase and stopper using the a stopper spacer. This procedure is described in detail in the chapter "Air calibration" in [MiPNet12.08].
See also: