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Difference between revisions of "Open chamber"

From Bioblast
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|abbr=O
|abbr=O
|description=The term "open O2k-Chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the [[Stopper-Spacer]].
|description=The term "open O2k-Chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the [[Stopper-Spacer]].
|info=
|info=[[System]]
}}
}}
== Application in [[HRR]] ==


:::: The liquid phase is defined as the system, which is then an [[open system]] with exchange of oxygen with the gas phase, finally resulting in a stable oxygen signal with a [[oxygen flux |slope over time]] of zero. Insert the stopper fully, siphon off any liquid from the receptacle of the stopper, and remove the stopper to a position defined by [[Stopper-Spacer]] to include an optimum volume of the gas phase obove the stirred liquid phase.
:::: The liquid phase is defined as the experimental [[system]], which is then an [[open system]] with exchange of oxygen with the gas phase, finally resulting in a stable oxygen signal with a [[oxygen flux |slope over time]] of zero. Insert the stopper fully, siphon off any liquid from the receptacle of the stopper, and remove the stopper to a position defined by the [[Stopper-Spacer]] to include an optimum volume of the gas phase above the stirred liquid phase.


:::: See also »[[Closed chamber]]
::::» ''Compare'': [[Closed chamber]]




{{Template:Keywords: Oxygen signal}}
{{Template:Keywords: Oxygen signal}}
{{Template:Oroboros QM}} [[Gnaiger Erich]] 2020-09-07

Revision as of 10:11, 7 September 2020


                  


O2k-Open Support

Open chamber



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

The term "open O2k-Chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the Stopper-Spacer.

Abbreviation: O

Reference: System

Application in HRR

The liquid phase is defined as the experimental system, which is then an open system with exchange of oxygen with the gas phase, finally resulting in a stable oxygen signal with a slope over time of zero. Insert the stopper fully, siphon off any liquid from the receptacle of the stopper, and remove the stopper to a position defined by the Stopper-Spacer to include an optimum volume of the gas phase above the stirred liquid phase.
» Compare: Closed chamber



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Quality management

Oroboros quality management from the O2k to NextGen-O2k
Gnaiger Erich 2020-09-07