Difference between revisions of "O2k-Virtual Support self-study material"

Revision as of 11:31, 7 March 2022

O2k-Virtual Support self-study material

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

General information

To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

O2k-Manual: O2k-FluoRespirometer
O2k-Manual: POS Service
Step 1: O2k-Videosupport: OroboPOS from Perspex housing
Step 2: O2k-Videosupport: Cathode cleaning
Step 3: O2k-Videosupport: Anode cleaning
Step 4: O2k-Videosupport: Membrane mounting
Step 5: O2k-Videosupport: DL7 Start DatLab7
Step 6: O2k-Videosupport: DL7 Insert OroboPOS
Step 7: O2k-Videosupport: Insert O2k Chamber
Step 8: O2k-Videosupport: O2k Chamber volume calibration

Part 1.2: DatLab

Part 1.3: O₂ calibration and instrumental background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

NextGen-O2k KOL Training

(R) These materials will be sent upon registration

PB-Module Manual (R)

O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)

DL-Protocol: SUIT-030_PB_ce_D069 (R)
O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)

DL-Protocol: SUIT-030_PB_ce_D070 (R)

Q-Module

Q-Module manual: MiPNet24.12 NextGen-O2k: Q-Module
CV software manual: MiPNet24.16 DatLab8.0: CV-Module
Videos: Q-Module

@@ Line 28: / Line 28: @@
 :::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
 :::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]]
-:::* [https://www.youtube.com/watch?v=onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/onOIVpmOEoE]] [https://youtu.be/onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]
 :::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]
@@ Line 40: / Line 40: @@
 :::* [[Mitochondrial preparations]]
 :::* [[MiPNet17.03 Shredder vs Fibres|Shredder ''vs'' Fibers]]
-:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/EjQGUr5M8v0]]  [https://youtu.be/EjQGUr5M8v0 O2k-Videosupport: Shredder tissue homogenate]
-:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/iN38rOkiBUQ]]  [https://youtu.be/iN38rOkiBUQ O2k-Videosupport: Fiber permeabilization]
 :::* [[Cell count and normalization in HRR]]
-:::* [https://www.youtube.com/watch?v=GX_SvnVGXgg O2k-Videosupport: Addition of biological sample to the respirometer chamber]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/GX_SvnVGXgg]]  [https://youtu.be/GX_SvnVGXgg O2k-Videosupport: Addition of biological sample to the respirometer chamber]
 '''SUIT protocols, SUITbrowser, DL-Protocols:'''
 :::* [[MitoPedia: SUIT]]
 :::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser]
-:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/8T33sp9KkJk]]  [https://youtu.be/8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)]
-:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/Vd66dHx-MyI]]  [https://youtu.be/Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)]
 === Part 2.2: Biological experiment and data analysis ===
 :::* [[MiPNet19.14 SOP Hamilton_microsyringes|SOP: Hamilton microsyringes]]
 :::* [[MiPNet09.12 O2k-Titrations|O2k-Titrations]]
-:::* [https://www.youtube.com/watch?v=9j4ZtHmHiss  O2k-Videosupport:  How to perform an experiment with a SUIT DL-Protocol (DLP)]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/9j4ZtHmHiss]]  [https://youtu.be/9j4ZtHmHiss  O2k-Videosupport:  How to perform an experiment with a SUIT DL-Protocol (DLP)]
 :::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen flux analysis]]
-:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/HRAGvmaK01I]]  [https://youtu.be/HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]
@@ Line 62: / Line 62: @@
 ''' O2k-Applications: Simultaneous  determination  of  O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes'''
 :::* [[Amplex UltraRed]]
-:::* [https://www.youtube.com/watch?v=nlVLXcbBRqs O2k-Videosupport: Smart Fluo-Sensor assembly]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/nlVLXcbBRqs]]  [https://youtu.be/nlVLXcbBRqs O2k-Videosupport: Smart Fluo-Sensor assembly]
-:::* [https://www.youtube.com/watch?v=eUukvgAi1yY O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/eUukvgAi1yY]]  [https://youtu.be/eUukvgAi1yY O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor]
 :::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]]
 :::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]]
-:::* [https://www.youtube.com/watch?v=_Fv0VSA_c14 O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
+:::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/_Fv0VSA_c14]]  [https://youtu.be/_Fv0VSA_c14 O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]