O2k-Coaching Days Innsbruck
| Description |  By organizing MitoFit Coaching Days Innsbruck, the OROBOROS-team responds to increasing demands for training in high-resolution respirometry (HRR) and O2k-Fluorometry beyond the capacity of our regular O2k-Workshops. MitoFit Coaching Days are offered in the OROBOROS MitoFit Laboratory according to demand for four to six participants. Experts provide training for quality control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high quality as required for a data base on mitochondrial physiology. The flexible programme is adjusted to the individual requirements of the participants, with specific coaching blocks particularly on instrumental setup and maintenance, instrumental quality control, MitoFit proficiency testing using standardized mitochondrial reference samples, DatLab analysis, SUIT protocols and experimental design, normalization of flux and data reporting, O2k-Fluorometry for measurement of hydrogen peroxide, measurement of mitochondrial membrane potential with fluorometric and potentiometric methods, measurement of respiration and acidification, quality control management in HRR. The MitoFit Coaching Days help participants to get prepared for their projects on HRR, even if the regular O2k-Workshops are fully booked. |
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| Product ID | 7#### |
| Type | O2k, Catalogue |
| Link | Next OROBOROS O2k-Workshops |
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MitoFit Coaching Days - Basic
- MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
MitoFit Coaching Days - Proficiency test
- MitoFit Coaching Days - Proficiency test provides training on high-resolution respirometry at a basic and advanced level, guiding through standard operating procedures at the instrumental and experimental level on mitochondrial respiratory function in intact and permeabilized cells. Each participant carries out with an individual Oxygraph-2k the instrumental assembly, oxygen sensor service, quality control with the O2 sensor test and O2k-chamber test, performs experiments with intact and permeabilized cells (MitoFit-Reference sample) using the SUIT reference protocol, completes DatLab analysis and summarizes statistically the results in comparison with the data of other participants and a controlled reference data set. Upon successful completion of the MitoFit proficiency test, a unique certificate on 'Quality control in high-resolution respirometry' will be obtained.
MitoFit Coaching Days - O2k-MultiSensor
- MitoFit Coaching Days - O2k-MultiSensor provides advanced training on O2k-Fluorometry for measurement of mt-membrane potential, H2O2 production, phosphorylation of ADP to ATP, and O2k-potentiometric measurement of mt-membrane potential with TPP+. O2k-MultiSensor protocols and applications are always performed in combination with respirometry. Discussion of experimental results, reporting, and interpretation of SUIT protocols constitutes an integral part of the training.
