Difference between revisions of "NextGen-O2k Scientific developments"

Latest revision as of 13:23, 3 April 2022

2022

NextGen-O2k Scientific developments

The revolutionary all-in-one instrument to conquer mitochondrial disease.

WP2: Scientific developments

Abstract

The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.

Determination of optimal Q-sensor measurement conditions
Determination of optimal measurement conditions for the PB-Module
Development of protocols for scientific, pre-clinical and algal biotechnology applications
Data handling and quality control

Achievements

Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.

Project Year 2: During the second project year, measurement conditions and protocols were optimized for the PB- and Q-Modules with a variety of biological samples. Additionally, the NADH-Module for evaluation of the NADH-pool in physio-pathological conditions was integrated in the NextGen-O2k. Testing of modules for the DatLab8 software were conducted and improvements applied. Moreover, SUIT protocols for general, pre-clinical, and algal biotechnology applications were developed. Quality control was maintained during the second period of project, an important factor in the development of the NexGen-O2k instrument and relevant protocols.

Progress and next steps

See » Track record

Luiza Cardoza, PhD. testing the Q-Module and DatLab8
Microalgae for testing PB module

NextGen-O2k MitoFit Preprints

Link	View	Reference	Year
Donnelly 2023 MitoFit	MitoFit Preprints 2023.2. Functional hypoxia reduces mitochondrial calcium uptake.	Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 — 2024-11-17 published in Redox Biol.	2023
Went 2021 MitoFit PB	MitoFit Preprints 2021.05.	Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005	2021
Huete-Ortega 2020 MitoFit Preprint Arch EA	MitoFit Preprint Arch EA20.1. High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations	Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.0001	2020

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+{{Template:NextGen-O2k header page name logo}}
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+<br />
+<big><big><big>'''WP2: Scientific developments'''</big></big></big>
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 == Abstract ==
-:::: To test measurement conditions and consequent adjustments. Develop protocols for (1) scientific and pre-clinical applications (mitochondrial physiology of non-photosynthetic cells) and (2) scientific algal biotechnological applications (chloroplasts and mitochondrial physiology of photosynthetic cells -microalgae and cyanobacteria).
+:::: The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
+::::* Determination of optimal Q-sensor measurement conditions
+::::* Determination of optimal measurement conditions for the PB-Module
+::::* Development of protocols for scientific, pre-clinical and algal biotechnology applications
+::::* Data handling and quality control
+== Achievements ==
+::::'''Project Year 1:'''  During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.
+::::'''Project Year 2:''' During the second project year, measurement conditions and protocols were optimized for the PB- and Q-Modules with a variety of biological samples. Additionally, the NADH-Module for evaluation of the NADH-pool in physio-pathological conditions was integrated in the NextGen-O2k. Testing of modules for the DatLab8 software were conducted and improvements applied. Moreover, SUIT protocols for general, pre-clinical, and algal biotechnology applications were developed. Quality control was maintained during the second period of project, an important factor in the development of the NexGen-O2k instrument and relevant protocols.
 == Progress and next steps ==
+See » [[Talk:NextGen-O2k Scientific developments|Track record]]
+<br/>
+<gallery mode=default perrow=4 widths="350px" heights="300px">
+File:Luiza Q-module testing and DatLab8.jpg|[[Cardoso Luiza HD|Luiza Cardoza, PhD.]] testing the Q-Module and DatLab8
+File:Microalgae for testing PB module.jpg|Microalgae for testing PB module
+|-
+</gallery>
+== NextGen-O2k MitoFit Preprints ==
+{{#ask:[[Instrument and method::NextGen-O2k]] [[Was published in journal::MitoFit Preprint Arch]]
+| mainlabel=Link
+|?Has info=View
+|?Has title=Reference
-== Links and references ==
+|?Was published in year=Year
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Was published in year
+|order=descending
+}}