Difference between revisions of "NextGen-O2k Documentation"

Latest revision as of 13:23, 3 April 2022

2022

NextGen-O2k Documentation

The revolutionary all-in-one instrument to conquer mitochondrial disease.

WP3: Documentation

Abstract

In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.

Achievements

Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.

Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published.

Progress and next steps

See » Track record

NextGen-O2k Videosupport clips

Q-Module (NextGen-O2k - DatLab 7)

Q-service

Assembly of the reference electrode
NG Polishing glassy carbon and platinum electrodes
NG O2k-Chamber volume calibration with Q-Sensor

Q-experiments

NG Q-experiments

Cleaning Q-Sensors

NG Cleaning after cyclic voltammetry
NG Cleaning after Q-experiments

FluoRespirometry

Smart Fluo-Sensor assembly
Exchanging a filter set of the smart-fluo sensor
H₂O₂ flux analysis with DatLab 7.4

O2k-Mitochondrial preparations

Shredder tissue homogenate
Fiber permeabilization
Addition of biological sample to the respirometer chamber

Quality Control

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration

NextGen-O2k trailers

NextGen-O2k: the Q-Module
NextGen-O2k: the NADH-Module
NextGen-O2k: the PQ-Module

NextGen-O2k demo

NextGen-O2k demo: Coenzyme Q redox state and mt-respiration
NextGen-O2k demo: mt-respiration and NADH
NextGen-O2k demo: mt-respiration and H₂O₂
NextGen-O2k demo: H₂O₂ calibration

NextGen-O2k Manuals

» NextGen-O2k manual

» DatLab8.0: CV-Module

» NextGen-O2k: Q-Module

» NextGen-O2k: NADH-Module

» NextGen-O2k: PB-Module

» O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8

» Oxygen flux_analysis - DatLab 7.4

» Safranin Analysis Template

» General template for mt-membrane potential analysis

» H₂O₂ flux analysis

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-{{MitoFit highlights page name}}
+{{Oroboros highlights page name}}
 {{Template:NextGen-O2k header page name logo}}
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@@ Line 14: / Line 14: @@
 :::: In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.
+== Achievements ==
+::::'''Project Year 1:''' In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
+::::'''Project Year 2:''' Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published.
 == Progress and next steps ==
-:::: '''2019-12-06:'''	First version of the CV-Manual released [[MiPNet24.16 DatLab8.0: CV-Module]]
+:::: See » [[Talk:NextGen-O2k Documentation|Track record]]
-:::: '''2019-11-22:'''	First draft of NextGen-O2k Manual/SOP finalized
+=== [[Image:PlayVideo.jpg|50px|link=https://wiki.oroboros.at/index.php/O2k-Videosupport]] NextGen-O2k Videosupport clips  ===
-:::: '''2019-10-21:''' 	Videos for our software DatLab 7.4 are released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#DatLab_7.4 O2k-Videosupport DatLab 7.4]
-:::: '''2019-09-24:'''	Videos for mitochondrial preparations are released – check them out: [https://wiki.oroboros.at/index.php/O2k-Videosupport#O2k-Mitochondrial_preparations O2k-Videosupport Mitochondrial preparations]
-:::: '''2019-09-20:''' 	First videos for the Q-Module are released – check them out: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport Q-Module]
-:::: '''2019-09-02:''' 	Recording of videos for our NextGen-O2k
-:::: '''2019-06-17:'''	Our Marketing Thaumaturge [[Cocco Paolo|Paolo Cocco]] participates at IOC139 Schroecken AT - to start with the recording of the first videos
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible mw-collapsed">
+'''Q-Module (NextGen-O2k - DatLab 7)'''
-== Links and references ==
+<div class="mw-collapsible-content">
+* '''Q-service'''
+[https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be  Assembly of the reference electrode]<br>
+[https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]<br>
+[https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]<br>
+---------
+* ''' Q-experiments'''
+[https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
+---------
+* ''' Cleaning Q-Sensors'''
+[https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]<br>
+[https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
+</div>
+</div>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible mw-collapsed">
+'''FluoRespirometry'''
+<div class="mw-collapsible-content">
+[https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]<br>
+[https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the smart-fluo sensor]<br
+>[https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
+</div>
+</div>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible mw-collapsed">
+'''O2k-Mitochondrial preparations'''
+<div class="mw-collapsible-content">
+[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]<br>
+[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]<br>
+[https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
+</div>
+</div>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible mw-collapsed">
+'''Quality Control'''
+<div class="mw-collapsible-content">
+[https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
+</div>
+</div>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible mw-collapsed">
+'''NextGen-O2k trailers'''
+<div class="mw-collapsible-content">
+[https://www.youtube.com/watch?v=MQGa_xMkyA4 NextGen-O2k: the Q-Module]<br>
+[https://www.youtube.com/watch?v=2UQ4ffzUz_o NextGen-O2k: the NADH-Module]<br>
+[https://www.youtube.com/watch?v=UEpuz7mbbBQ NextGen-O2k: the PQ-Module]<br>
+</div>
+</div>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible mw-collapsed">
+'''NextGen-O2k demo'''
+<div class="mw-collapsible-content">
+* NextGen-O2k demo: Coenzyme Q redox state and mt-respiration<br>
+* NextGen-O2k demo: mt-respiration and NADH<br>
+* NextGen-O2k demo: mt-respiration and H<sub>2</sub>O<sub>2</sub><br>
+* NextGen-O2k demo: H<sub>2</sub>O<sub>2</sub> calibration<br>
+</div>
+</div>
+=== [[Image:O2k-Manual.jpg|50px|link=https://wiki.oroboros.at/index.php/O2k-Manual]] NextGen-O2k Manuals ===
+::: » [[MiPNet26.13_NextGen-O2k_manual|NextGen-O2k manual]]
+::: » [[MiPNet24.16 DatLab8.0: CV-Module|DatLab8.0: CV-Module]]
+::: » [[MiPNet24.12 NextGen-O2k: Q-Module |NextGen-O2k: Q-Module]]
+::: » [[MiPNet26.12 NextGen-O2k: NADH-Module|NextGen-O2k: NADH-Module]]
+::: » [[MiPNet26.11 NextGen-O2k: PB-Module|NextGen-O2k: PB-Module]]
+::: » [[MiPNet06.03 POS-calibration-SOP|O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8]]
+::: » [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| Oxygen flux_analysis - DatLab 7.4]]
+::: » [[MiPNet24.08 Safranin Analysis Template| Safranin Analysis Template]]
+::: » [[MiPNet24.09 General Template for Mt-membrane Potential Analysis| General template for mt-membrane potential analysis]]
+::: » [[MiPNet24.10 H2O2 flux analysis| H<sub>2</sub>O<sub>2</sub> flux analysis]]