Difference between revisions of "N-junction"
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{{MitoPedia | {{MitoPedia | ||
|description=[[File:SUIT-Catg N.jpg|right|300px|N-junction]] | |description=[[File:SUIT-Catg N.jpg|right|300px|N-junction]] | ||
The '''N-junction''' is a junction for [[convergent electron flow]] in the [[electron transfer system]] (ETS) through the mt-[[NADH]] pool to [[Complex I]] (CI), and further transfer through the [[Q-junction]] to [[Complex III]] (CIII). Representative [[Substrate types |type N substrates]] are pyruvate, glutamate and malate. The corresponding dehydrogenases (PDH, GDH, MDH) generate NADH, the substrate of [[Complex I]] (CI). The concept of the N-junction and [[F-junction]] provides a basis for defining [[categories of SUIT protocols]]. | The '''N-junction''' is a junction for [[convergent electron flow]] in the [[electron transfer system]] (ETS) from [[N]]-type substrates through the mt-[[NADH]] pool to [[Complex I]] (CI), and further transfer through the [[Q-junction]] to [[Complex III]] (CIII). Representative [[Substrate types |type N substrates]] are pyruvate, glutamate and malate. The corresponding dehydrogenases (PDH, GDH, MDH) generate NADH, the substrate of [[Complex I]] (CI). The concept of the N-junction and [[F-junction]] provides a basis for defining [[categories of SUIT protocols]]. | ||
|info=[[Gnaiger 2014 MitoPathways]] | |info=[[Gnaiger 2014 MitoPathways]] | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
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|mitopedia O2k and high-resolution respirometry=SUIT protocol | |mitopedia O2k and high-resolution respirometry=SUIT protocol | ||
}} | }} | ||
Β Contributed by [[Gnaiger E]] 2016-02-12 | Β Contributed by [[Gnaiger E]] 2016-02-12 |
Revision as of 22:45, 20 March 2016
Description
The N-junction is a junction for convergent electron flow in the electron transfer system (ETS) from N-type substrates through the mt-NADH pool to Complex I (CI), and further transfer through the Q-junction to Complex III (CIII). Representative type N substrates are pyruvate, glutamate and malate. The corresponding dehydrogenases (PDH, GDH, MDH) generate NADH, the substrate of Complex I (CI). The concept of the N-junction and F-junction provides a basis for defining categories of SUIT protocols.
Reference: Gnaiger 2014 MitoPathways
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
Contributed by Gnaiger E 2016-02-12