Difference between revisions of "Myxothiazol"
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::::'''Preparation of 1 mM storage and stock solution''' (dissolved in | ::::'''Preparation of 1 mM storage and stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': | ||
::::# Weigh 1 mg of myxothiazol into a small glass vial. | ::::# Weigh 1 mg of myxothiazol into a small glass vial. | ||
::::# Add 2.05 mL ethanol. | ::::# Add 2.05 mL ethanol. | ||
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::::'''Preparation of 0.25mM stock solution''' (dissolved in | ::::'''Preparation of 0.25mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''': | ||
::::# Take 100 µL of the 1 mM stock solution. | ::::# Take 100 µL of the 1 mM stock solution. | ||
::::# Add 300 µL ethanol. | ::::# Add 300 µL ethanol. | ||
Revision as of 11:55, 22 June 2021
- high-resolution terminology - matching measurements at high-resolution
Myxothiazol
Description
Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
Abbreviation: Myx
Reference: MiPNet09.12
Application in HRR
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 °C, CAS: 76706-55-3, M = 487.7 g·mol-1
- Hazard statements: H300; fatal if swallowed
- Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 °C, CAS: 76706-55-3, M = 487.7 g·mol-1
- Available again from Sigma with this new product number. The old product number was M 5779.
- Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Weigh 1 mg of myxothiazol into a small glass vial.
- Add 2.05 mL ethanol.
- Divide into 0.2 mL (use glass vials).
- Store at -20 °C.
- Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- »O2k manual titrations MiPNet09.12
- Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 0.5 µM.
- »O2k manual titrations MiPNet09.12
- Preparation of 0.25mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Take 100 µL of the 1 mM stock solution.
- Add 300 µL ethanol.
- Preparation of 0.25mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- »O2k manual titrations MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 0.5 µM.
- »O2k manual titrations MiPNet09.12
Compare
- Antimycin A is less expensive than Myx but induces higher ROS production.
MitoPedia topics:
Inhibitor
