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Difference between revisions of "MitoFit"

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{{MitoFit page name}}
{{MitoFit page name}}
:::: <big><big>mitochondria fit & well</big></big>
:::: <big><big>The Open Access preprint server for mitochondrial physiology and bioenergetics</big></big>
== MitoFit Preprint in focus ==
<br />


[[File:MitoFit Preprint Arch pdf.png|left|160px|link=http://www.mitofit.org/images/d/dc/Schoepf_2019_MitoFit_Preprint_Arch.pdf |MitoFit pdf]]  <big><big>'''[http://www.mitofit.org/images/d/dc/Schoepf_2019_MitoFit_Preprint_Arch.pdf OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature]'''</big></big>
::::» Schöpf B, Weissensteiner H, SchÀfer G, Fazzini F, Charoentong P, Naschberger A, Rupp B, Fendt L, Bukur V, Eichelbrönner I, Sorn P, Sahin U, Kronenberg F, Gnaiger E, Klocker H (2019) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature. MitoFit Preprint Arch doi:10.26124/mitofit:190003. - [[Schoepf 2019 MitoFit Preprint Arch |»Bioblast link«]]
<br />


[[Image:Logo MitoFit-OROBOROS.jpg|right|300px|MitoFit]]
[[Image:MitoFit Preprint Arch.png|left|120px|link=MitoFit Preprint Arch]]
'''Diagnosis of mitochondrial functions and mitochondrial injuries'''
== New MitoFit Preprints ==
:::: Diagnosis of mitochondrial functions and mitochondrial injuries represents a current challenge for biomedical research and development. We address these challenges by introducing and extending the approach of '''[[cell ergometry]]''' as the state-of-the-art functional test of mitochondrial competence.  ''MitoFit'' establishes an inter-university Network in Mitochondrial Physiology and Comprehensive Exercise Testing, supporting translational research on mitochondrial competence in close collaboration with the local industry and other networks, e.g. Oncotyrol. '''''Mitochondrial fitness''''' will play an increasing role in therapeutic and preventive medicine, with exercise and caloric balance providing the most effective measures for the reduction of several age-related health risks and degenerative diseases. MitoFit is a gateway and milestone to better diagnose, support and treat patients in our modern, rapidly ageing society.
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::::» [[K-Regio MitoFit |K-Regio project MitoFit]]
::::» [[OROBOROS MitoFit Laboratory]]
::::» [[MitoFit news]] 


== Preprints for [[Gentle Science]] ==
{{MitoFit preprint}}


== MitoFit - hot topics ==
[[Category:Mitochondrial Global Network]]
::::» [[MitoFit Science Camp 2016 Kuehtai AT]]
 
::::» [[MITOEAGLE |COST Action approved: MITOEAGLE]]
 
::::* MitoFit quality control - new on MitoPedia
::::# [[MitoPedia: Sample preparations]]
::::# [[MitoPedia: SUIT]]
::::» [https://www.nia.nih.gov/research/blog/2015/12/making-molecular-map-exercise Making a molecular map of exercise - NIH Common Fund]
 
 
== Concept ==
 
:::: A physically active and nutritionally healthy life style induces regulatory mechanisms that maintain aerobic performance and support functional mitochondrial competence. Even in advancing age, exercise increases aerobic fitness and maintains muscle mitochondrial density above the sedentary benchmark, contributing to the high quality of life characteristic of alpine regions such as Tyrol. Functional mitochondrial competence and aerobic fitness reduce several age-related health risks, including cardiovascular and degenerative diseases, such as type 2 diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer. As a result of a sedentary life style and progression to obesity, the decline of muscle mitochondrial capacity may contribute to accelerated ageing processes and development of degenerative diseases. Diagnostic evaluation of '''functional mitochondrial competence''' requires '''scientific and technological mitochondrial competence'''. '''MitoFit fit&well''' will combine the specialized scientific focus on mitochondrial research and exercise testing with the expertise of local companies of world-wide leadership in this expanding field. Beyond the added value emanating from the combination and coordination of these diagnostic elements, the consortium will collaborate on the implementation of refined and novel diagnostic protocols as a milestone towards an internationally recognized centre of excellence, with an explicit focus on sport and clinical perspectives to address mitochondrial health and disease.
 
=== Objectives ===
 
:::: MitoFit combines the expertise of experienced partners in a unique corporate framework. MitoFit will establish a research and diagnostic laboratory network to form a '''Centre of Excellence in Mitochondrial Physiology, Pathology, Comprehensive Exercise Testing, and Genetic Fingerprinting'''. Diagnostic analyses include cardiopulmonary (VO2max; CPX) and metabolic (31P-NMR; Ergospect) exercise testing, evaluation of mitochondrial performance (high-resolution respirometry; OROBOROS INSTRUMENTS), and genetic screening (mtDNA haplotypes, relevant nuclear encoded genes). Refined state-of-the-art diagnostic protocols are extended and evaluated in a training programme under acute high-altitude hypoxia versus normoxic conditions with healthy control subjects. Exposure to acute hypoxia represents a typical condition for millions of individuals annually skiing or mountaineering in Austria and particularly in Tyrol. Comprehensive mitochondrial and exercise testing protocols will be developed and validated in these studies. Case studies will translate the scientific expertise into the clinical setting. As a result, MitoFit will offer and apply these diagnostic services for (i) monitoring of training programmes in competitive athletes, (ii) life style analyses of the general population, (iii) exercise interventions for preventive and therapeutic medicine, and (iv) clinical diagnosis of individual patients.
 
=== Technological and scientific innovation ===
 
:::: Diagnosis of mitochondrial function has advanced to a new technological level by the introduction of high-resolution respirometry, and on a scientific level of innovation by multiple substrate-uncoupler-inhibitor titration protocols with permeabilized fibres from small muscle biopsies. Nevertheless, compared to the small needle biopsy, functional mt-diagnosis on far less invasive blood samples (thrombocytes) might provide a '''new perspective for large-scale screening''' of individual athletes, extended cohorts in clinical studies, and application for individual patients. Simultaneous measurement of mitochondrial respiration and additional parameters (mt-membrane potential by ion selective electrodes, hydrogen peroxide production by spectrofluorimetry coupled to the O2k) requires the development of new diagnostic protocols that can be applied to permeabilized muscle fibres and/or intact and permeabilized thrombocytes. Although conventional cardiopulmonary exercise testing (CPX) provides insights into the coupling of external to cellular respiration, it mirrors insufficiently the metabolic processes in the contracting and recovering muscle. Thus, combining CPX and techniques assessing bioenergetic processes in the working muscles will undoubtedly increase the conclusiveness of exercise testing in athletes and patients. These diagnostic MultiSensor protocols in combination with comprehensive exercise testing and genetic fingerprinting will generate an '''innovative repertoire of diagnostic tests''' that can be offered in the context of sports and mitochondrial diseases. 
 
 
== From ''MitoCom'' to '''MitoFit''' – 2020 m and above ==
:::: Join those who seek the special recreational and adventurous experience with physical exercise in the alps. Your exercise is the gateway to your mitochondrial fitness.
 
:::: '''MitoFit''' evolves as a unique network of practitioners in the high-end sector of recreation, sport and protective medicine, in collaboration with experts on mitochondrial physiology and mitochondrial medicine.
 
:::: We all know that feeling of being well from head to toe – or from muscle to brain – up in the mountains. Scientists want to get to the basis of this wellbeing, to gain insights into invisible molecular functions:  is it caused by the ’good air‘ we breathe, even though air gets very thin high up in the Alps? How do the lungs, blood, heart, brain, arms and legs – all organs and all somatic cells – react to altitude and relaxation in nature, to challenges in sport or to a short period of life on a mountain pasture?
 
:::: Mitochondria are responsible for the energy supply in human and animal tissues; this is [[cell ergometry]], cell respiration – mitochondrial oxygen consumption. With the [[Oxygraph-2k |OROBOROS O2k]], we determine energy turnover of carbohydrates and fatty acids and quantify the efficiency of mitochondria in maintaining the vital body functions. In order to determine cellular fitness, parameters for oxidative stress and stress resistance are measured along with mitochondrial oxygen consumption. Biomedical research all over the world applies this method for the diagnosis of mitochondrial competence, especially in sports medicine and gerontology. The O2k, developed by the company OROBOROS INSTRUMENTS, is the world-wide leading instrument for diagnosing the aerobic capacity of mitochondria with the help of small samples of tissue or blood. OROBOROS INSTRUMENTS is located in Innsbruck, Austria. In the Mitochondrial Research Laboratory, the company tests the newest developments and develops standard protocols in cell ergometry.
 
 
 
[[Image:MitoCom Tyrol.jpg|left|80px|link=MitoCom O2k-Fluorometer|MitoCom]]
:::: The logo of ''MitoCom Tyrol'' (now transformed to MitoFit) is based on ''MiPArt'' by [http://www.mipart.at/?Odra-Noel Odra Noel]. ''Further details:'' »[[MitoCom O2k-Fluorometer]].
 
 
=== Impact on life style: Tyrol – a heart for sport ===
 
:::: Mitochondrial knowledge will, with its innovative approach, arouse a new awareness of preventive medicine, to which a physically active and calorie-balanced lifestyle can contribute significantly. Of particular importance is “healthy aging”, a generally improved quality of life and a reduced risk of a multiplicity of degenerative diseases influenced by the mitochondria: e.g. obesity, diabetes 2, various forms of cancer and dementias. Mitochondrial competence will be made a reality to a larger proportion of the population and the regional identity strengthened both amongst our own population and amongst tourists through the offer of exercise-orientated leisure packages in the Alpine landscape of Tyrol.
 
[[File:MitoFit-protective medicine.jpg|700px|right|Instrumental innovation and interdisciplinary cooperation in mitochondrial physiology lead to new perspectives of functional mitochondrial diagnosis. Check your mitochondrial health is conveyed to the public as a keyword for taking care of a physically active life style as an invaluable component of preventive and therapeutic medicine.]]
 
:::: Linkages are being created by '''MitoFit''' between research and clinical/practical needs with regard to the very recently established Olympic centre at the Institute of Sport Science of the LFU in Innsbruck and the Altitude Training Centre in KĂŒhtai near Innsbruck leading to optimization of diagnostic exercise testing and training strategies. Importantly, this project seeks to promote research on exercise effects at moderate altitude. Tyrol represents a unique location which is visited by several millions of skiers and mountaineers, annually. On the one hand, regular physical activity and probably also exposures to moderate altitudes up to about 2500 m may contribute to well-being and longevity. On the other hand, however, unaccustomed exercise and acute altitude exposure may trigger serious cardiovascular adverse events in subjects at risk. This initiative should contribute to a better understanding of mechanisms responsible for beneficial and adverse effects supporting the development of appropriate preventive measures.
 
:::: Appreciation by politicians and communication with an aspirational general public are essential to benefit from world-class research, for comprehensive mitochondrial and exercise testing and implementation of exercise as a medicine, and the development of a scientific foundation that may allow us to translate the complex diagnostic results into a patient-related mitochondrial score. The MitoFit network will emphasize the connections to international mitochondrial networks, for exchange of expertise and standardization of diagnostic approaches, in a joint effort to helping mitochondrial patients, preventing numerous diseases and making a difference to society.
 
 
[[Image:MitoFit.jpg|left|60px|link=http://www.mitofit.org/index.php/K-Regio MitoFit|K-Regio MitoFit]]
'''See also'''
:::* '''[[K-Regio MitoFit]]''' is the next step following the K-Regio project ''MitoCom'' (mitochondrial competence; [[MitoCom_O2k-Fluorometer|O2k-Fluorometer]]), extending our world-wide technological leadership in mitochondrial respirometry with the development of a quality control system, MitoFit-Knowledge Management Platform and applications in preventive medicine (K-Regio project MitoFit).
:::* Health tourism: http://www.tt.com/wirtschaft/7750785-91/mehr-gesundheit-f%C3%BCr-tirol-urlauber.csp
:::* [http://www.uibk.ac.at/ipoint/dossiers/forschung_im_fokus/983677.html Sport science in focus]

Revision as of 20:55, 12 June 2019


MitoFit Preprints         MitoFit Preprints        
Gnaiger 2019 MitoFit Preprints
       
Gnaiger MitoFit Preprints 2020.4
        MitoFit DOI Data Center         MitoPedia: Preprints         Bioenergetics Communications


MitoFit

The Open Access preprint server for mitochondrial physiology and bioenergetics

MitoFit Preprint in focus


MitoFit pdf

OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature

» Schöpf B, Weissensteiner H, SchÀfer G, Fazzini F, Charoentong P, Naschberger A, Rupp B, Fendt L, Bukur V, Eichelbrönner I, Sorn P, Sahin U, Kronenberg F, Gnaiger E, Klocker H (2019) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature. MitoFit Preprint Arch doi:10.26124/mitofit:190003. - »Bioblast link«


MitoFit Preprints.png

New MitoFit Preprints

 ViewReferencePublished
Aasander Frostner 2022 MitoFitMitoFit Preprints 2022.14.
MitoFit pdf
Towards a treatment for mitochondrial disease: current compounds in clinical development
Åsander Frostner E, SimĂłn Serrano S, Chamkha I, Donnelly E, ElmĂ©r E, Hansson MJ (2022) Towards a treatment for mitochondrial disease: current compounds in clinical development. https://doi.org/10.26124/mitofit:2022-0014 — 2022-06-28 published in Bioenerg Commun 2022.4.2022
Abed Rabbo 2022 MitoFitMitoFit Preprints 2022.05.
MitoFit pdf
NUBPL: a mitochondrial Complex I deficiency disorder
Abed Rabbo M, Stiban J (2022) NUBPL: a mitochondrial Complex I deficiency disorder. https://doi.org/10.26124/mitofit:2022-0005 — 2022-06-28 published in Bioenerg Commun 2022.3.2022
Alencar 2022 MitoFitMitoFit Preprints 2022.09.v2.
MitoFit pdf
A unifying hypothesis for the extraordinary energy metabolism of bloodstream Trypanosoma brucei
Alencar MB, Ramos EV, Silber AM, Zíková A, Oliveira MF (2022) The extraordinary energy metabolism of the bloodstream Trypanosoma brucei forms: a critical review and a hypothesis. https://doi.org/10.26124/mitofit:2022-0009.v2 — 2022-12-05 published in Bioenerg Commun 2022.17.2022
Arias-Reyes 2023 MitoFitMitoFit Preprints 2023.6.
MitoFit pdf
Mitochondrial plasticity in the retrosplenial cortex enhances ATP synthesis during acclimatization to hypoxia in mice but not in rats.
Arias-Reyes C, Aliaga-RaduĂĄn F, Pinto-Aparicio R, Joseph V, Soliz J (2023) Mitochondrial plasticity in the retrosplenial cortex enhances ATP synthesis during acclimatization to hypoxia in mice but not in rats. MitoFit Preprints 2023.6. https://doi.org/10.26124/mitofit:2023-00062023
Baglivo 2022 MitoFit-QCMitoFit Preprints 2022.18.
MitoFit pdf
Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry »Watch the presentation«
Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 — 2022-08-04 published in Bioenerg Commun 2022.8.2022
Balbaisi 2022 MitoFitMitoFit Preprints 2022.03.
MitoFit pdf
Barth Syndrome: A Genetic Ailment with a Lipid Component and Bioenergetic Ramifications
Balbaisi A, Stiban J (2022) Barth Syndrome: a genetic ailment with a lipid component and bioenergetic ramifications. https://doi.org/10.26124/mitofit:2022-0003 — 2022-06-28 published in Bioenerg Commun 2022.5.2022
Bellissimo 2022 MitoFitMitoFit Preprints 2022.26.
MitoFit pdf
The influence of adenylate cycling on mitochondrial calcium-induced permeability transition pore in permeabilized skeletal muscle fibres.
Bellissimo CA, Sondergaard S, Hughes MC, Ramos SV, Larsen S, Perry CGR (2022) The influence of adenylate cycling on mitochondrial calcium-induced permeability transition pore in permeabilized skeletal muscle fibres. https://doi.org/10.26124/mitofit:2022-0026 — 2023-02-20 published in Bioenerg Commun 2023.1.2022
Bombaca 2022 MitoFitMitoFit Preprints 2022.16.
MitoFit pdf
Mitochondrial plasticity in trypanosomatids as a stress adaptation mechanism
Bombaça ACS, Menna-Barreto RFS (2022) Mitochondrial plasticity in trypanosomatids as a stress adaptation mechanism. https://doi.org/10.26124/mitofit:2022-0016 — 2022-12-23 published in Bioenerg Commun 2022.20.2022
Brown 2020 MitoFit Preprint ArchMitoFit Preprint Arch 2020.1
MitoFit pdf
Vitamin D deficiency: a factor in COVID-19 progression severity and mortality? An urgent call for research - 2020-02-29 Published without peer-review in »Modern Ghana«
Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. https://doi.org/10.26124/mitofit:2000012020
Brunetta 2024 MitoFitMitoFit Preprints 2024.1.
MitoFit pdf
Methodological considerations for the determination of mitochondrial ADP sensitivity in skeletal muscle
Brunetta HS, Holloway GP (2024) Methodological considerations for the determination of mitochondrial ADP sensitivity in skeletal muscle. MitoFit Preprints 2024. 1. https://doi.org/10.26124/mitofit:2024-00012024
Cabral-Costa 2022 MitoFitMitoFit Preprints 2022.27.
MitoFit pdf
Mitochondrial Ca2+ handling as a cell signaling hub: lessons from astrocyte function.
Cabral-Costa JV, Kowaltowski AJ (2022) Mitochondrial Ca2+ handling as a cell signaling hub: lessons from astrocyte function. https://doi.org/10.26124/mitofit:2022-00272022
Cardoso 2021 MitoFit MgGMitoFit Preprints 2021.01.
MitoFit pdf
Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration
Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. https://doi.org/10.26124/mitofit:2021-0001 — 2021-06-30 published in Bioenerg Commun 2021.1.2021
Cecatto 2023 MitoFitrat and Drosophila brain
ischemia and aging models]

MitoFit Preprints 2023.10.
MitoFit pdf
Fatty acid ÎČ-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse
Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka M, Liepinsh E (2023) Fatty acid ÎČ-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Preprints 2023.10. https://doi.org/10.26124/mitofit:2023-00102023
Chabi 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.1
MitoFit pdf
[https://www.mitofit.org/images/5/57/Chabi_2019_MitoFit_Preprint_Arch_doi_10.26124mitofitea19.mitoeagle.0001.pdf Generating reference values on mitochondrial respiration in permeabilized muscle fibers
Chabi BĂ©atrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, WĂŒst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM (2019) Generating reference values on mitochondrial respiration in permeabilized muscle fibers. https://doi.org/10.26124/mitofit:ea19.MitoEAGLE.00012019
Chicco 2022 MitoFitMitoFit Preprints 2022.17.
MitoFit pdf
Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols »Watch the presentation«
Chicco AJ, Zilhaver PT, Whitcomb LA, Fresa KJ, Izon CS, Gonzalez-Franquesa A, Izon CS, Dometita C, Irving BA, Garcia-Roves PM (2022) Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols. https://doi.org/10.26124/mitofit:2022-00172022
Crispim 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.7.v2
MitoFit pdf
Effects of atovaquone and 4-nitrobenzoate on Plasmodium falciparum respiration
Crispim M, Verdaguer IB, Zafra CA, Katzin AM (2019) Effects of atovaquone and 4-nitrobenzoate on Plasmodium falciparum respiration. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0007.v22019
Dambrova 2022 MitoFitMitoFit Preprints 2022.20.
MitoFit pdf
Mitochondrial metabolites acylcarnitines: therapeutic potential and drug targets »Watch the presentation«
Dambrova M, Cecatto C, Vilskersts R, Liepinsh E (2022) Mitochondrial metabolites acylcarnitines: therapeutic potential and drug targets. https://doi.org/10.26124/mitofit:2022-0020 2022-11-25 published in Bioenerg Commun 2022.15.2022
Davis 2023 MitoFitMitoFit Preprints 2023.5.
MitoFit pdf
Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration.
Davis MS, Barrett MR, Bayly WM, Bolinger A (2023) Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration. MitoFit Preprints 2023.5. https://doi.org/10.26124/mitofit:2023-00052023
Di Marcello 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.5.v3
MitoFit pdf
Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism
Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M (2019) Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0005.v32019
Doerrier 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.9
MitoFit pdf
Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers
Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00092019
Donnelly 2022 MitoFit HypoxiaMitoFit Preprints 2022.25.v2.
MitoFit pdf
The ABC of hypoxia – what is the norm »Watch the Bioblast 2022 presentation«
Donnelly C, Schmitt S, Cecatto C, Cardoso LHD, Komlodi T, Place N, Kayser B, Gnaiger E (2022) The ABC of hypoxia – what is the norm. https://doi.org/10.26124/mitofit:2022-0025.v2 — 2022-11-14 published in Bioenerg Commun 2022.12.2022
Donnelly 2023 MitoFitMitoFit Preprints 2023.2.
MitoFit pdf
Functional hypoxia reduces mitochondrial calcium uptake.
Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 — 2024-11-17 published in Redox Biol.2023
Fischer 2021 MitoFit Fe liverMitoFit Preprints 2021.09.
MitoFit pdf
Dietary iron overload and Hfe-/- related hemochromatosis alter hepatic mitochondrial function
Fischer C, Volani C, Komlódi T, Seifert M, Demetz E, Valente de Souza L, Auer K, Petzer V, von Raffay L, Moser P, Gnaiger E, Weiss G (2021) Dietary iron overload and Hfe-/- related hemochromatosis alter hepatic mitochondrial function. https://doi.org/10.26124/mitofit:2021-0009 — 2021-11-16 published in Antioxidants2021
Fischer 2022 MitoFit FeMitoFit Preprints 2022.02.
MitoFit pdf
Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver
Fischer C, Valente de Souza L, Komlódi T, Garcia-Souza LF, Volani C, Tymoszuk P, Demetz E, Seifert M, Auer K, Hilbe R, Brigo N, Petzer V, Asshoff M, Gnaiger E, Weiss G (2022) Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver. https://doi.org/10.26124/mitofit:2022-0002 — 2022-03-21 published in Metabolites2022
Gainutdinov 2022 MitoFitMitoFit Preprints 2022.15.v.2
MitoFit pdf
Impaired Ca2+ signalling as an indicator of disturbed mitochondrial function in fibroblasts from patients with sporadic and familial ALS
Gainutdinov T, Debska-Vielhaber G, Gizatullina Z, Vielhaber S, Orynbayeva Z, Gellerich FN (2022) Impaired Ca2+ signalling as an indicator of disturbed mitochondrial function in fibroblasts from patients with sporadic and familial ALS. https://doi.org/10.26124/mitofit:2022-0015.v2 — 2022-12-09 published in Bioenerg Commun 2022.18.2022
Ganguly 2022 MitoFitMitoFit Preprints 2022.13.v2.
MitoFit pdf
Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a complex interplay of cell death pathways
Ganguly U, Bir A, Chakrabarti S (2022) Cytotoxicity of mitochondrial Complex I inhibitor rotenone: a complex interplay of cell death pathways. https://doi.org/10.26124/mitofit:2022-0013.v2 — 2022-11-22 published in Bioenerg Commun 2022.14.2022
Garcia Menendez 2023 MitoFitMitoFit Preprints 2023.8.
MitoFit pdf
Update and a new statement of the electromagnetic coupling hypothesis to explain the translocation of protons in mitochondria
bacteria and chloroplasts.

Garcia Menendez R (2023) Update and a new statement of the electromagnetic coupling hypothesis to explain the translocation of protons in mitochondria, bacteria and chloroplasts. MitoFit Preprints 2023.8. https://doi.org/10.26124/mitofit:2023-00082023
Gnaiger 2019 MitoFit PreprintsMitoFit Preprint Arch 2019.1.v6
MitoFit pdf
Mitochondrial respiratory states and rates



Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. https://doi.org/10.26124/mitofit:190001.v6. — Published: 2020-05-20 Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v12019
Gnaiger 2019 MitoFit Preprints EditorialMitoFit Preprint Arch 2019.2.v2.
MitoFit pdf
Editorial: A vision on preprints



Gnaiger E (2019) Editorial: A vision on preprints for mitochondrial physiology and bioenergetics. https://doi.org/10.26124/mitofit:190002.v22019
Gnaiger 2020 MitoFit xMitoFit Preprints 2020.04.v2.
MitoFit pdf
The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed.
Gnaiger E (2021) The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v22021
Gnaiger 2021 MitoFit BCAMitoFit Preprints 2021.08.
MitoFit pdf
Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts
Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.08. https://doi.org/10.26124/mitofit:2021-00082021
Gnaiger 2023 MitoFit CIIMitoFit Preprints 2023.3.v6.
MitoFit pdf
Complex II ambiguities ― FADH2 in the electron transfer system
Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. MitoFit Preprints 2023.3.v6. https://doi.org/10.26124/mitofit:2023-0003.v6 - Published 2023-11-22 J Biol Chem (2024)2023
Gonçalves 2019 Mitofit Preprint Arch EAMitoFit Preprint Arch EA19.2.
MitoFit pdf
Aging effects on mitochondrial control factors in Pink1 knockout Drosophila melanogaster
Gonçalves Debora F, Courtes AA, Hartmann DD, Carvalho PR, Marques DM, Machado ML, Furtado AV, Soares FA, Dalla Corte CL (2019) Aging effects on mitochondrial control factors in Pink1 knockout Drosophila melanogaster. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00022019
Gross 2023 MitoFitMitoFit Preprints 2023.9.
MitoFit pdf
A BID-MTCH2 love story: Energizing mitochondria until apoptosis do them part?
Gross A (2023) A BID-MTCH2 love story: Energizing mitochondria until apoptosis do them part? MitoFit Preprints 2023.9. https://doi.org/10.26124/mitofit:2023-0009 — 2024-02-08 published in Bioenerg Commun 2024.1.2023
Hassan 2020 MitoFit Preprint ArchMitoFit Preprint Arch 2020.3
MitoFit pdf
Alterations in mitochondrial respiratory capacity and membrane potential: a link between mitochondrial dysregulation and autism. -
Hassan Hazirah, Gnaiger Erich, Zakaria Fazaine, Makpol Suzana, Abdul Karim Norwahidah (2020) Alterations in mitochondrial respiratory capacity and membrane potential: a link between mitochondrial dysregulation and autism. https://doi.org/10.26124/mitofit:2000032020
Heichler 2022 MitoFitMitoFit Preprints 2022.06.
MitoFit pdf
Evaluation of hepatotoxic effects of acetaminophen on HepG2 cells by parallel real-time monitoring in a multi-sensor analysis platform for automated cell-based assays
Heichler C, Nagy M, Wolf P (2022) Evaluation of hepatotoxic effects of acetaminophen on HepG2 cells by parallel real-time monitoring in a multi-sensor analysis platform for automated cell-based assays. https://doi.org/10.26124/mitofit:2022-0006 — 2022-07-06 published in Bioenerg Commun 2022.6.2022
Heimler 2022 MitoFitMitoFit Preprints 2022.07.
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Platelet bioenergetics associated with resting metabolic rate and exercise capacity in older women
Heimler SR, Phang HJ, Bergstrom J, Mahapatra G, Dozier S, Gnaiger E, Molina AJA (2022) Platelet bioenergetics are associated with resting metabolic rate and exercise capacity in older women. https://doi.org/10.26124/mitofit:2022-0007 — 2022-06-28 published in Bioenerg Commun 2022.2.2022
Holzner 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.4.
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Evidence of altered mitochondrial function in glycolytic but not oxidative skeletal muscle in mice after one month of high fat high sucrose feeding
Holzner Lorenz MW, Sowton AP, Murray AJ (2019) Evidence of altered mitochondrial function in glycolytic, but not oxidative skeletal muscle in mice after one month of high fat, high sucrose feeding. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00042019
Huete-Ortega 2020 MitoFit Preprint Arch EAMitoFit Preprint Arch EA20.1.
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High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations
Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.00012020
Ingram 2020 MitoFit Preprint ArchMitoFit Preprint Arch 2020.2
MitoFit pdf
Sex differences characterise inflammatory profiles of cerebellar mitochondria and are attenuated in Parkinson’s disease. -
Ingram TL, Shephard F, Sarmad S, Ortori CA, Barrett DA, Chakrabarti L (2020) Sex differences characterise inflammatory profiles of cerebellar mitochondria and are attenuated in Parkinson’s disease. https://doi.org/10.26124/mitofit:2000022020
Jusic 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.6.
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Noncoding RNAs regulatory network in mitochondria
Jusic Amela, Hajrulahovic A, Devaux Y (2019) Noncoding RNAs regulatory network in mitochondria. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00062019
Karavyraki 2022 MitoFitMitoFit Preprints 2022.22.
MitoFit pdf
A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells »Watch the presentation«
Karavyraki M, Gnaiger E, Porter RK (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. https://doi.org/10.26124/mitofit:2022-0022 — 2022-11-11 published in Bioenerg Commun 2022.11.2022
Komlodi 2021 MitoFit AmR-O2MitoFit Preprints 2021.10.
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Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed
Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/mitofit:2021-0010 — 2021-12-21 published in Bioenerg Commun 2021.4.2021
Komlodi 2021 MitoFit QMitoFit Preprints 2021.02.v4.
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Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria
Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/mitofit:2021-0002.v4 — 2021-11-11 published in Bioenerg Commun 2021.3.2021
Komlodi 2022 MitoFitMitoFit Preprints 2022.12.
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The protonmotive force - not merely membrane potential »Watch the presentation«
Komlódi T, Tretter L (2022) The protonmotive force – not merely membrane potential. https://doi.org/10.26124/mitofit:2022-0012 — 2022-11-29 published in Bioenerg Commun 2022.16.2022
Krako Jakovljevic 2021 MitoFit PDMitoFit Preprints 2021.04.
MitoFit pdf
Mitochondrial homeostasis in cellular models of Parkinson’s Disease
Krako Jakovljevic N, Ebanks B, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. https://doi.org/10.26124/mitofit:2021-0004 — 2021-10-06 published in Bioenerg Commun 2021.2.2021
Machado 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.8
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Interplay between metformin and miR-378a-3p in cells under hyperglycaemia
Machado Ivo F, Teodoro JS, Palmeira CM, Rolo AP (2019) Interplay between metformin and miR-378a-3p in cells under hyperglycaemia. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00082019
Moreno-Sanchez 2022 MitoFitMitoFit Preprints 2022.24.
MitoFit pdf
Estimation of energy pathway fluxes in cancer cells- beyond the Warburg effect
Moreno-SĂĄnchez R, Robledo-Cadena DX, Pacheco-VelĂĄzquez SC, RodrĂ­guez-EnrĂ­quez S (2022) Estimation of energy pathway fluxes in cancer cells- beyond the Warburg effect. https://doi.org/10.26124/mitofit:2022-00242022
Mutschler 2022 MitoFitMitoFit Preprints 2022.10.
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Membranes as therapeutic targets - liposomes as therapeutic options
Mutschler R (2022) Membranes as therapeutic targets - liposomes as therapeutic options. https://doi.org/10.26124/mitofit:2022-00102022
Oliveira 2023 MitoFitMitoFit Preprints 2023.1.
MitoFit pdf
Boosting mitochondrial metabolism research in South America: building a case for a local Drosophila stock center.
Oliveira MT, Couto-Lima CA (2023) Boosting mitochondrial metabolism research in South America: building a case for a local Drosophila stock center. https://doi.org/10.26124/mitofit:2023-00012023
Oliveira 2023 MitoFit SpotlightMitoFit Preprints 2023.7.
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A navigation chart to avoid turbulence in Drosophila mitochondrial research.
Oliveira MF, Aveiro Y, Couto-Lima CA (2023) A navigation chart to avoid turbulence in Drosophila mitochondrial research. MitoFit Preprints 2023.7. https://doi.org/10.26124/mitofit:2023-0007 — 2023-11-06 published in Bioenerg Commun 2023.4.2023
Pallag 2022 MitoFit ProlineMitoFit Preprints 2022.01.v3.
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Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I
Pallag G, Nazarian S, Ravasz D, Bui D, Komlódi T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I. https://doi.org/10.26124/mitofit:2022-0001.v3 — 2022-05-04 published in https://doi.org/10.3390/ijms230951112022
Posch 2022 MitoFitMitoFit Preprints 2022.04.
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How to optimize respiratory models for SARS-CoV-2 research »Watch the presentation«
Posch W, Dichtl S, Zaderer V, Lass-Flörl C, Wilflingseder D (2022) How to optimize respiratory models for SARS-CoV-2 research. https://doi.org/10.26124/mitofit:2022-0004 — 2022-09-08 published in Bioenerg Commun 2022.9.2022
Roach 2022 MitoFitMitoFit Preprints 2022.23.v2.
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Chlorophyll fluorescence of Chlamydomonas reinhardtii; insights into the complexities
Roach T (2022) Chlorophyll fluorescence of Chlamydomonas reinhardtii; insights into the complexities. https://doi.org/10.26124/mitofit:2022-0023.v2 — 2022-11-10 published in Bioenerg Commun 2022.10.2022
Rodriguez 2023 MitoFitMitoFit Preprints 2023.4.
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Harmonising protocols to measure Drosophila respiratory function in mitochondrial preparations.
Rodríguez E, Bettinazzi S, Inwongwan S, Camus MF, Lane N (2023) Harmonising protocols to measure Drosophila respiratory function in mitochondrial preparations. MitoFit Preprints 2023.4. https://doi.org/10.26124/mitofit:2023-0004 — 2023-08-01 published in Bioenerg Commun 2023.3.2023
Schoepf 2019 MitoFit Preprint ArchMitoFit Preprint Arch 2019.3.
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OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature
Schöpf B, Weissensteiner H, SchÀfer G, Fazzini F, Charoentong P, Naschberger A, Rupp B, Fendt L, Bukur V, Eichelbrönner I, Sorn P, Sahin U, Kronenberg F, Gnaiger E, Klocker H (2019) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature. https://doi.org/10.26124/mitofit:190003 - 2020-03-20 published in »Nature Communications 11:1487«2019
Serna 2022 MitoFitMitoFit Preprints 2022.21.
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Measuring mitochondrial Ca2+ efflux in isolated mitochondria and permeabilized cells
Serna JDC, Ramos VM, Cabral-Costa JV, Vilas-Boas EA, Amaral AG, Ohya G, da Silva CCC, Kowaltowski AJ (2022) Measuring mitochondrial Ca2+ efflux in isolated mitochondria and permeabilized cells. https://doi.org/10.26124/mitofit:2022-0021 — 2022-07-28 published in Bioenerg Commun 2022.7.2022
Torres-Quesada 2022 MitoFitMitoFit Preprints 2022.08.
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Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines
Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines. https://doi.org/10.26124/mitofit:2022-0008 — 2022-08-13 published in https://doi.org/10.3390/cancers141639172022
Torres-Quesada 2022 MitoFit KinaseMitoFit Preprints 2022.11.
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Kinase perturbations redirect mitochondrial function »Watch the presentation«
Torres-Quesada O, Strich S, Stefan E (2022) Kinase perturbations redirect mitochondrial function. https://doi.org/10.26124/mitofit:2022-0011 — 2022-11-15 published in Bioenerg Commun 2022.13.2022
Umbrasas 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.1.
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Itaconic acid decreases mitochondrial respiration and ROS generation in brain tissue
Umbrasas Danielius, Vanagas T, Cizas P, Borutaite V (2019) Itaconic acid decreases mitochondrial respiration and ROS generation in brain tissue. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00012019
Vera Vives 2022 MitoFitMitoFit Preprints 2022.19.
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The robustness of NextGen-O2k for building PI curves in microalgae »Watch the presentation«
Vera-Vives AM, Perin G, Morosinotto T (2022) The robustness of NextGen-O2k for building PI curves in microalgae. https://doi.org/10.26124/mitofit:2022-0019 — 2022-12-15 published in Bioenerg Commun 2022.19.2022
Vernerova 2021 MitoFit PLTMitoFit Preprints 2021.06.
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Mitochondrial respiration of platelets: comparison of isolation methods
Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O (2021) Mitochondrial respiration of platelets: comparison of isolation methods. https://doi.org/10.26124/mitofit:2021-0006 — 2021-12-08 published in Biomedicines2021
Went 2021 MitoFit PBMitoFit Preprints 2021.05.
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Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-00052021
Zdrazilova 2021 MitoFit ace-sceMitoFit Preprints 2021.07.
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Comparable respiratory activity in attached and suspended human fibroblasts
Zdrazilova L, Hansikova H, Gnaiger E (2021) Comparable respiratory activity in attached and suspended human fibroblasts. https://doi.org/10.26124/mitofit:2021-0007 — 2022-03-03 published in PLOS ONE 17:e02644962021
Zuccolotto-dos-Reis 2021 MitoFit ACoARMitoFit Preprints 2021.03.
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Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease
Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. https://doi.org/10.26124/mitofit:2021-0003 - 2021-09 published in »European Journal of Clinical Investigation 51(9):e13574«2021
Zujovic 2019 MitoFit Preprint Arch EAMitoFit Preprint Arch EA19.3.
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Palmitate treated human hepatocellular carcinoma HuH7 cells require higher digitonin concentration for plasma membrane permeabilization
Zujovic Tijana, Krako Jakovljevic N, Pavlovic K, Markovic I, Lalic NM (2019) Palmitate treated human hepatocellular carcinoma HuH7 cells require higher digitonin concentration for plasma membrane permeabilization. https://doi.org/10.26124/mitofit:ea19.MiPSchool.00032019


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