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MitoEAGLE preprint 2017-09-21

From Bioblast


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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


MitoEAGLE preprint 2017-09-21


Publications in the MiPMap
MitoEAGLE preprint 2018-02-18(21) The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21.

ยป Bioblast pdf - ยป Versions -- Next step ยป MitoEAGLE preprint 2018-02-08

MitoEAGLE (2018) MitoEAGLE preprint

Abstract: Note: Subscript โ€˜ยงโ€™ indicates throughout the text those parts, where potential differences provide a mathematically correct but physicochemically incomplete description and should be replaced by stoichiometric potential differences (Gnaiger 1993b). A unified concept on vectorial motive transformations and scalar chemical reactions will be derived elsewhere (Gnaiger, in prep.). Appreciation of the fundamental distinction between differences of potential versus differences of stoichiometric potential may be considered a key to critically evaluate the arguments presented in Section 3 on the protonmotive force. Since this discussion appears to be presently beyond the scope of a MitoEAGLE position statement, Section 3 will be removed from the next version and final manuscript. This section should become a topic of discussion within Working Group 1 of the MitoEAGLE consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. โ€ข Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux

Fig. 1. The oxidative phosphorylation (OXPHOS) system. Electron transfer pathways (A) are coupled to the phosphorylation pathway (B).
Fig. 2. The proton circuit and coupling in oxidative phosphorylation (OXPHOS). Modified after Gnaiger 2014 MitoPathways.

Preprints for Gentle Science

Citation:
MitoEAGLE preprint 2017-09-21(Version 21). The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21

Authors

Contributing co-authors: Confirming to have read the final manuscript, possibly to have made additions or suggestions for improvement, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
Supporting co-authors: Confirming to have read the final manuscript, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)


Building blocks of mitochondrial physiology MitoEAGLE recommendations Part 1.
Phase 3:  ยป MitoEAGLE preprint 2018-02-08
Phase 2: ยป MitoEAGLE preprint 2017-09-21
Phase 1: ยป Discussion (44 versions)
Section 2: Oxidative phosphorylation and coupling states in mitochondrial preparations
Fig. 3. Mechanisms of respiratory uncoupling.
Fig. 7. Four-compartmental model of oxidative phosphorylation with respiratory states (ET, OXPHOS, LEAK) and corresponding rates (E, P, L). Modified from Gnaiger (2014).

Table Coupling states.png Table Coupling terms.png Table Chance states.png


Section 3: The protonmotive force and proton flux
Table Protonmotive force matrix.png
Fig. 8. Three formats of the protonmotive unit (A) and protonmotive force (B).
Table Physical constants.png

Table Power, exergy, force, flux, advancement.png


Section 4: Normalization: fluxes and flows
Fig. 9. Different meanings of rate may lead to confusion, if the normalization is not sufficiently specified.
Fig. 10. Mitochondrial recovery, YmtE, in preparation of isolated mitochondria.


Table Sample concentrations and normalization of flux.png
Fig. 11. Structure-function analysis of performance of an object X (an organism, organ or tissue, or a cell). O2 flow, IX,O2, is the product of performance per functional element (element function, mitochondria-specific flux), element density (mitochondrial density, DmtE), and size of the object X (mass MX).

Table Sample types.png


Labels: MiParea: Respiration, mt-Awareness 



Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria  Enzyme: Marker enzyme  Regulation: Coupling efficiency;uncoupling, Flux control, mt-Membrane potential, Uncoupler  Coupling state: LEAK, OXPHOS, ET  Pathway: ROX