Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

MitoEAGLE preprint 2017-09-21

From Bioblast
Revision as of 10:26, 27 January 2018 by Gnaiger Erich (talk | contribs)


MiPsociety
News and Events        
BEC 2020.1 Mitochondrial physiology
       
MitoEAGLE
        Working Groups         Short-Term Scientific Missions         Management Committee         Members        
MitoGlobal
   


EU-logo.jpg

COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


MitoEAGLE preprint 2017-09-21


Publications in the MiPMap
MitoEAGLE preprint 2018-01-27(20) The protonmotive force and respiratory control: Building blocks of mitochondrial physiology Part 1. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21.

» Last update 2018-01-27: Bioblast pdf - » Versions

Corresponding author:, Gnaiger E, Contributing co-authors:, Ahn B, Alves MG, Amati F, Aral C, Arandarcikaite O, Asander Frostner E, Bailey DM, Bastos Sant'Anna Silva AC, Battino M, Beard DA, Ben-Shachar D, Bishop D, Breton S, Brown GC, Brown RA, Buettner GR, Calabria E, Cardoso LHD, Carvalho E, Casado Pinna M, Cervinkova Z, Chang SC, Chicco AJ, Chinopoulos C, Coen PM, Collins JL, Crisostomo L, Davis MS, Dias T, Distefano G, Doerrier C, Drahota Z, Duchen MR, Ehinger J, Elmer E, Endlicher R, Fell DA, Ferko M, Ferreira JCB, Filipovska A, Fisar Z, Fisher J, Garcia-Roves PM, Garcia-Souza LF, Genova ML, Gonzalo H, Goodpaster BH, Gorr TA, Grefte S, Han J, Harrison DK, Hellgren KT, Hernansanz P, Holland O, Hoppel CL, Houstek J, Hunger M, Iglesias-Gonzalez J, Irving BA, Iyer S, Jackson CB, Jansen-Duerr P, Jespersen NR, Jha RK, Kaambre T, Kane DA, Kappler L, Karabatsiakis A, Keijer J, Keppner G, Komlodi T, Kopitar-Jerala N, Krako Jakovljevic N, Kuang J, Kucera O, Labieniec-Watala M, Lai N, Laner V, Larsen TS, Lee HK, Lemieux H, Lerfall J, Lucchinetti E, MacMillan-Crow LA, Makrecka-Kuka M, Meszaros AT, Michalak S, Moisoi N, Molina AJA, Montaigne D, Moore AL, Mracek T, Muntane J, Muntean DM, Murray AJ, Nedergaard J, Nemec M, Newsom S, Nozickova K, O'Gorman D, Oliveira PF, Oliveira PJ, Orynbayeva Z, Pak YK, Palmeira CM, Patel HH, Pecina P, Pereira da Silva Grilo da Silva F, Pesta D, Petit PX, Pichaud N, Pirkmajer S, Porter RK, Pranger F, Prochownik EV, Puurand M, Radenkovic F, Reboredo P, Renner-Sattler K, Robinson MM, Rohlena J, Roesland GV, Rossiter HB, Rybacka-Mossakowska J, Salvadego D, Scatena R, Schartner M, Scheibye-Knudsen M, Schilling JM, Schlattner U, Schoenfeld P, Scott GR, Shabalina IG, Shevchuk I, Siewiera K, Singer D, Sobotka O, Spinazzi M, Stankova P, Stier A, Stocker R, Sumbalova Z, Suravajhala P, Tanaka M, Tandler B, Tepp K, Tomar D, Towheed A, Tretter L, Trivigno C, Tronstad KJ, Trougakos IP, Tyrrell DJ, Urban T, Velika B, Vendelin M, Vercesi AE, Victor VM, Villena JA, Wagner BA, Ward ML, Watala C, Wei YH, Wieckowski MR, Wohlwend M, Wolff J, Wuest RCI, Zaugg K, Zaugg M, Zorzano A, Supporting co-authors:, Bakker BM, Bernardi P, Boetker HE, Borsheim E, Borutaite V, Bouitbir J, Calbet JA, Calzia E, Chaurasia B, Clementi E, Coker RH, Collin A, Das AM, De Palma C, Dubouchaud H, Durham WJ, Dyrstad SE, Engin AB, Fornaro M, Gan Z, Garlid KD, Garten A, Genova ML, Gourlay CW, Granata C, Haas CB, Haavik J, Haendeler J, Hand SC, Hepple RT, Hickey AJ, Hoel F, Jang DH, Kainulainen H, Khamoui AV, Klingenspor M, Koopman WJH, Kowaltowski AJ, Krajcova A, Lane N, Lenaz G, Malik A, Markova M, Mazat JP, Menze MA, Methner A, Neuzil J, Oliveira MT, Pallotta ML, Parajuli N, Pettersen IKN, Porter C, Pulinilkunnil T, Ropelle ER, Salin K, Sandi C, Sazanov LA, Silber AM, Skolik R, Smenes BT, Soares FAA, Sokolova I, Sonkar VK, Swerdlow RH, Szabo I, Trifunovic A, Thyfault JP, Valentine JM, Vieyra A, Votion DM, Williams C, Zischka H (2017) MitoEAGLE preprint

Abstract: Clarity of concept and consistency of nomenclature are key trademarks of a research field. These trademarks facilitate effective transdisciplinary communication, education, and ultimately further discovery. As the knowledge base and importance of mitochondrial physiology to human health expand, the necessity for harmonizing nomenclature concerning mitochondrial respiratory states and rates has become increasingly apparent. Peter Mitchell’s chemiosmotic theory establishes the links between electrical and chemical components of energy transformation and coupling in oxidative phosphorylation. This unifying concept of the protonmotive force provides the framework for developing a consistent theory and nomenclature for mitochondrial physiology and bioenergetics. Herein, we follow IUPAC guidelines on general terms of physical chemistry, extended by considerations on open systems and irreversible thermodynamics. The protonmotive force is not a force as defined in physics. This conflict is resolved by the generalized formulation of isomorphic forces in energy transformations. We align the nomenclature of classical bioenergetics on respiratory states with a concept-driven constructive terminology to address the meaning of each respiratory state. Furthermore, we suggest uniform standards for the evaluation of respiratory states that will ultimately support the development of databases of mitochondrial respiratory function in species, tissues and cells studied under diverse physiological and experimental conditions. In this position statement, in the frame of COST Action MitoEAGLE, we endeavour to provide a balanced view on mitochondrial respiratory control, a fundamental introductory presentation of the concept of the protonmotive force, and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux Bioblast editor: Gnaiger E

Fig. 1. The oxidative phosphorylation (OXPHOS) system. Electron transfer pathways (A) are coupled to the phosphorylation pathway (B).
Fig. 2. The proton circuit and coupling in oxidative phosphorylation (OXPHOS). Modified after Gnaiger 2014 MitoPathways.

Preprints for Gentle Science

Citation:
MitoEAGLE preprint 2017-09-21(Version #). The protonmotive force and respiratory control: Building blocks of mitochondrial physiology Part 1. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21

Authors

Contributing co-authors: Confirming to have read the final manuscript, possibly to have made additions or suggestions for improvement, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
Supporting co-authors: Confirming to have read the final manuscript, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)

Discussion

» Talk:MitoEAGLE preprint 2017-09-21
» Phase 1

Concept: COST Action MitoEAGLE

  • Phase 1: 44 versions until 2017-09-18
  • Phase 2 (2017-09-21 ongoing): MitoEAGLE preprint - with updates on route to let the final publication fly. Feedback, suggestions, and confirmation from co-authors.
  • Phase 3: MiP2017/MitoEAGLE Hradec Kralove CZ - Discussion of manuscript submission for journal publication. Contact the editor(s) of our finally targeted journal(s), to obtain a first opinion if submission to this journal will be adequate. Cell Metabolism and BBA have been mentioned as possible preferences.
  • Phase 4: Manuscript submission to a preprint server, such as BioRxiv and a peer-reviewed open access journal that is indexed by The Web of Science and PubMed.
Section 2: Oxidative phosphorylation and coupling states in mitochondrial preparations
Fig. 3. Mechanisms of respiratory uncoupling.
Fig. 7. Four-compartmental model of oxidative phosphorylation with respiratory states (ET, OXPHOS, LEAK) and corresponding rates (E, P, L). Modified from Gnaiger (2014).

Table Coupling states.png Table Coupling terms.png Table Chance states.png


Section 3: The protonmotive force and proton flux
Table Protonmotive force matrix.png
Fig. 8. Three formats of the protonmotive unit (A) and protonmotive force (B).
Table Physical constants.png

Table Power, exergy, force, flux, advancement.png


Section 4: Normalization: fluxes and flows
Fig. 9. Different meanings of rate may lead to confusion, if the normalization is not sufficiently specified.
Fig. 10. Mitochondrial recovery, Ymte, in preparation of isolated mitochondria.


Table Sample concentrations and normalization of flux.png
Fig. 11. Structure-function analysis of performance of a sample entity X (an organism, organ or tissue, or a cell). O2 flow, IX,O2, is the product of performance per functional element (element function, mitochondria-specific flux), element density (mitochondrial density, Dmte), and size of entity X (mass MX).

Table Sample types.png


Labels: MiParea: Respiration, mt-Awareness 



Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria  Enzyme: Marker enzyme  Regulation: Coupling efficiency;uncoupling, Flux control, mt-Membrane potential, Uncoupler  Coupling state: LEAK, OXPHOS, ET  Pathway: ROX