Difference between revisions of "MitoEAGLE preprint 2017-09-21"
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|title=MitoEAGLE preprint 2018-02-06(21) The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21. | |title=MitoEAGLE preprint 2018-02-06(21) The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21. | ||
|info=<big></big> [[File:PDF.jpg|120px|link=http://www.mitoeagle.org/images/5/5b/MitoEAGLE_preprint_2017-09-21.pdf |Bioblast pdf]] - » [http://www.mitoeagle.org/index.php/File:MitoEAGLE_preprint_2017-09-21.pdf Versions] -- '''Next step » [[MitoEAGLE preprint 2018-02-08]]''' | |info=<big></big> [[File:PDF.jpg|120px|link=http://www.mitoeagle.org/images/5/5b/MitoEAGLE_preprint_2017-09-21.pdf |Bioblast pdf]] - » [http://www.mitoeagle.org/index.php/File:MitoEAGLE_preprint_2017-09-21.pdf Versions] -- '''Next step » [[MitoEAGLE preprint 2018-02-08]]''' | ||
|authors='''''Corresponding author:''''', Gnaiger E | |authors=MitoEAGLE, '''''Corresponding author:''''', Gnaiger E | ||
|year= | |year=2018 | ||
|journal=MitoEAGLE preprint | |journal=MitoEAGLE preprint | ||
|abstract= | |abstract='''Note''': Subscript ‘§’ indicates throughout the text those parts, where ''potential differences'' provide a mathematically correct but physicochemically incomplete description and should be replaced by ''stoichiometric potential differences'' ([[Gnaiger 1993 Pure Appl Chem |Gnaiger 1993b]]). A unified concept on vectorial motive transformations and scalar chemical reactions will be derived elsewhere (Gnaiger, in prep.). Appreciation of the fundamental distinction between ''differences of potential'' versus ''differences of stoichiometric potential'' may be considered a key to critically evaluate the arguments presented in Section 3 on the protonmotive force. Since this discussion appears to be presently beyond the scope of a MitoEAGLE position statement, Section 3 will be removed from the [[MitoEAGLE preprint 2018-02-08 |'''next version''']] and final manuscript. This section should become a topic of discussion within [[WG1 MitoEAGLE protocols, terminology, documentation |Working Group 1]] of the MitoEAGLE consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. | ||
'''Note''': Subscript ‘§’ indicates throughout the text those parts, where ''potential differences'' provide a mathematically correct but physicochemically incomplete description and should be replaced by ''stoichiometric potential differences'' ([[Gnaiger 1993 Pure Appl Chem |Gnaiger 1993b]]). A unified concept on vectorial motive transformations and scalar chemical reactions will be derived elsewhere (Gnaiger, in prep.). Appreciation of the fundamental distinction between ''differences of potential'' versus ''differences of stoichiometric potential'' may be considered a key to critically evaluate the arguments presented in Section 3 on the protonmotive force. Since this discussion appears to be presently beyond the scope of a MitoEAGLE position statement, Section 3 will be removed from the [[MitoEAGLE preprint 2018-02-08 |'''next version''']] and final manuscript. This section should become a topic of discussion within [[WG1 MitoEAGLE protocols, terminology, documentation |Working Group 1]] of the MitoEAGLE consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. | |||
|keywords=Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux | |keywords=Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux | ||
}} | }} | ||
[[File:OXPHOS system.png|right|400px|thumb|Fig. 1. The oxidative phosphorylation (OXPHOS) system. Electron transfer pathways (A) are coupled to the phosphorylation pathway (B).]] | [[File:OXPHOS system.png|right|400px|thumb|Fig. 1. The oxidative phosphorylation (OXPHOS) system. Electron transfer pathways (A) are coupled to the phosphorylation pathway (B).]] | ||
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'''Concept''': [[COST Action MitoEAGLE |COST Action MitoEAGLE]] | '''Concept''': [[COST Action MitoEAGLE |COST Action MitoEAGLE]] | ||
::::* Phase 1: [[The protonmotive force and respiratory control |44 versions until 2017-09-18]] | ::::* Phase 1: [[The protonmotive force and respiratory control |44 versions until 2017-09-18]] | ||
::::* | ::::* Phase 2.1 (2017-09-21): MitoEAGLE preprint - with updates on route to let the final publication fly. Feedback, suggestions, and confirmation from co-authors. | ||
::::* Phase | ::::* Phase 2.2: [[MiP2017/MitoEAGLE Hradec Kralove CZ]] - Discussion of manuscript submission for journal publication. | ||
::::* Phase 3 : (2018-02-08) [[MitoEAGLE preprint 2018-02-08]] - Contact the editor(s) of our finally targeted journal(s), to obtain a first opinion if submission to this journal will be adequate. Cell Metabolism and BBA have been mentioned as possible preferences. | |||
::::* Phase 4: Manuscript submission to a preprint server, such as [[BioRxiv]] and a peer-reviewed open access journal that is indexed by ''The Web of Science'' and ''PubMed''. | ::::* Phase 4: Manuscript submission to a preprint server, such as [[BioRxiv]] and a peer-reviewed open access journal that is indexed by ''The Web of Science'' and ''PubMed''. | ||
Revision as of 12:16, 8 February 2018
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE preprint 2017-09-21
MitoEAGLE preprint 2018-02-06(21) The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21. |
» - » Versions -- Next step » MitoEAGLE preprint 2018-02-08
MitoEAGLE, Corresponding author:, Gnaiger E (2018) MitoEAGLE preprint
Abstract: Note: Subscript ‘§’ indicates throughout the text those parts, where potential differences provide a mathematically correct but physicochemically incomplete description and should be replaced by stoichiometric potential differences (Gnaiger 1993b). A unified concept on vectorial motive transformations and scalar chemical reactions will be derived elsewhere (Gnaiger, in prep.). Appreciation of the fundamental distinction between differences of potential versus differences of stoichiometric potential may be considered a key to critically evaluate the arguments presented in Section 3 on the protonmotive force. Since this discussion appears to be presently beyond the scope of a MitoEAGLE position statement, Section 3 will be removed from the next version and final manuscript. This section should become a topic of discussion within Working Group 1 of the MitoEAGLE consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. • Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, chemiosmotic theory, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux
- Citation:
- MitoEAGLE preprint 2017-09-21(Version 21). The protonmotive force and respiratory control. - http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21
Authors
- Contributing co-authors: Confirming to have read the final manuscript, possibly to have made additions or suggestions for improvement, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
- Supporting co-authors: Confirming to have read the final manuscript, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials. (alphabetical, to be extended)
Discussion
Concept: COST Action MitoEAGLE
- Phase 1: 44 versions until 2017-09-18
- Phase 2.1 (2017-09-21): MitoEAGLE preprint - with updates on route to let the final publication fly. Feedback, suggestions, and confirmation from co-authors.
- Phase 2.2: MiP2017/MitoEAGLE Hradec Kralove CZ - Discussion of manuscript submission for journal publication.
- Phase 3 : (2018-02-08) MitoEAGLE preprint 2018-02-08 - Contact the editor(s) of our finally targeted journal(s), to obtain a first opinion if submission to this journal will be adequate. Cell Metabolism and BBA have been mentioned as possible preferences.
- Phase 4: Manuscript submission to a preprint server, such as BioRxiv and a peer-reviewed open access journal that is indexed by The Web of Science and PubMed.
Section 2: Oxidative phosphorylation and coupling states in mitochondrial preparations
Section 3: The protonmotive force and proton flux
Section 4: Normalization: fluxes and flows
Labels: MiParea: Respiration, mt-Awareness
Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria
Enzyme: Marker enzyme
Regulation: Coupling efficiency;uncoupling, Flux control, mt-Membrane potential, Uncoupler
Coupling state: LEAK, OXPHOS, ET
Pathway: ROX