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Difference between revisions of "MiPNet23.14 IOC135 Busan KR"

From Bioblast
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=== Local organizers ===
=== Local organizers ===
:::: [[Han J |Prof. Dr. Jin Han]] Β 
:::: [[Han J |Prof. Dr. Jin Han]] Β 
:::: [[Kim HK |Associate Prof. Dr. Hyoung Kyu Kim]]
:::::: [[Kim HK |Associate Prof. Dr. Hyoung Kyu Kim]]
[[KR Busan Han J]] - Oroboros O2k-Training Centre
:::::: username: @mitophysiol (telegram)
<br /><br />
:::::: https://t.me/mitophysiol
:::::: Phone: +82-51-890-8778
:::: Cardiovascular and Metabolic DiseaseΒ  Center (CMDC)
:::: Inje University
:::: Gaegume 2 dong, Busan, Korea
:::: [[KR Busan Han J]] - Oroboros O2k-Training Centre
<br />


=== Programme - with O2k demo experiment ===
=== Programme - with O2k demo experiment ===
::::* 14:00 Welcome
:::: 14:00 Welcome
[[Image:O2k-Titration Set.JPG|right|250px|link=O2k-Titration Set]]
[[Image:O2k-Titration Set.JPG|right|250px|link=O2k-Titration Set]]
::::* 14:15 Recent instrumental innovations
:::: 14:15 Recent instrumental innovations
::::::* [[O2k-FluoRespirometer]] - the concept
::::::* [[O2k-FluoRespirometer]] - the concept
::::::* [[Smart Fluo-Sensor]]s (O2k-Series H)
::::::* [[Smart Fluo-Sensor]]s (O2k-Series H)
Line 52: Line 58:


[[File:Protocol-menu.png|thumb|right|250px|link=Run_DL-Protocol/Set_O2_limit|'''Protocol menu''']]
[[File:Protocol-menu.png|thumb|right|250px|link=Run_DL-Protocol/Set_O2_limit|'''Protocol menu''']]
::::* 14:45 Start O2k demo experiment - [[SUIT-9 AmR pce D19]] (O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> flux with permeabilized cells)
:::: 14:45 Start O2k demo experiment - [[SUIT-9 AmR pce D19]] (O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> flux with permeabilized cells)


::::* 15:00 Innovations in DatLab 7 - and more in [[MitoPedia: DatLab]]
:::: 15:00 Innovations in DatLab 7 - and more in [[MitoPedia: DatLab]]
::::::* DatLab 7 and instrumental quality control: SOPs from cleaning to instrumental background test
::::::* DatLab 7 and instrumental quality control: SOPs from cleaning to instrumental background test
::::::* SUIT-Protocols Β» [[Run DL-Protocol/Set O2 limit]]
::::::* SUIT-Protocols Β» [[Run DL-Protocol/Set O2 limit]]
Line 60: Line 66:
::::::* New features of [[Mark statistics - DatLab |Mark statistics]]
::::::* New features of [[Mark statistics - DatLab |Mark statistics]]


::::* 16:00 Break
:::: 16:00 Break


::::* 16:30 Bioenergetic snapshot of mitochondrial fitness: Select the appropriate SUIT protocol Β 
:::: 16:30 Bioenergetic snapshot of mitochondrial fitness: Select the appropriate SUIT protocol Β 


::::* 18:00 DatLab Analysis: Evaluation of the O2k demo experiment
:::: 18:00 DatLab Analysis: Evaluation of the O2k demo experiment


::::* 18:30 DatLab Analysis: Flux per volume, flux per mass, flow per cell, flux control ratio, flux control factor
:::: 18:30 DatLab Analysis: Flux per volume, flux per mass, flow per cell, flux control ratio, flux control factor


::::* 19:45 Conclusions
:::: 19:45 Conclusions




=== Contact ===
=== Contact ===
Username: @mitophysiol (telegram)
:::: Hyoung Kyu Kim. PhD.
:::: Associate professor
:::: Username: @mitophysiol (telegram)
:::: https://t.me/mitophysiol
:::: Phone: +82-51-890-8778


https://t.me/mitophysiol
:::: μΈμ œλŒ€ν•™κ΅ μ‹¬ν˜ˆκ΄€λŒ€μ‚¬μ§ˆν™˜μ„Όν„° λΆ€κ΅μˆ˜
Β 
:::: κΉ€ν˜•κ·œ 올림
Phone: +82-51-890-8778





Revision as of 10:27, 31 October 2018

                



MiPNet23.14 IOC135 Busan KR

Publications in the MiPMap
O2k-Network

ASMRM 2018, Busan KR, 2018 Nov 06 to 07. Oroboros for MIRACLE - the bioenergetic snapshot of mitochondrial fitness (IOC135).

Β» ASMRM 2018 Busan KR

Oroboros (2018-11-06) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

Β» ASMRM 2018 Busan KR


β€’ O2k-Network Lab: AT Innsbruck Oroboros

O2k-FluoRespirometer


O2k-Workshop

Basic and advanced applications of the Oroboros O2k-FluoRespirometer

Time

Nov 6, 14:00-20:00

Location

O2k-Network
National Research Laboratory for Mitochondrial Signaling
Department of Physiology
Inje College of Medicine
Cardiovascular and Metabolic Disease Center
Inje University
Gaegume 2 dong, Busan, South Korea
Address
3rd Floor, Experimental Room
Bokjiro 75, Busan Jin Gu, Busan City, Korea
https://goo.gl/maps/JATb11LcY5N2


SmartFluo.png
Jin Han

Local organizers

Prof. Dr. Jin Han
Associate Prof. Dr. Hyoung Kyu Kim
username: @mitophysiol (telegram)
https://t.me/mitophysiol
Phone: +82-51-890-8778
Cardiovascular and Metabolic Disease Center (CMDC)
Inje University
Gaegume 2 dong, Busan, Korea
KR Busan Han J - Oroboros O2k-Training Centre


Programme - with O2k demo experiment

14:00 Welcome
O2k-Titration Set.JPG
14:15 Recent instrumental innovations
Protocol menu
14:45 Start O2k demo experiment - SUIT-9 AmR pce D19 (O2 and H2O2 flux with permeabilized cells)
15:00 Innovations in DatLab 7 - and more in MitoPedia: DatLab
16:00 Break
16:30 Bioenergetic snapshot of mitochondrial fitness: Select the appropriate SUIT protocol
18:00 DatLab Analysis: Evaluation of the O2k demo experiment
18:30 DatLab Analysis: Flux per volume, flux per mass, flow per cell, flux control ratio, flux control factor
19:45 Conclusions


Contact

Hyoung Kyu Kim. PhD.
Associate professor
Username: @mitophysiol (telegram)
https://t.me/mitophysiol
Phone: +82-51-890-8778
μΈμ œλŒ€ν•™κ΅ μ‹¬ν˜ˆκ΄€λŒ€μ‚¬μ§ˆν™˜μ„Όν„° λΆ€κ΅μˆ˜
κΉ€ν˜•κ·œ 올림


IOC recommended reading

Β» IOC recommended reading


ASMRM luncheon seminar by Oroboros

Erich Gnaiger, PhD., Oroboros Instruments, CEO
Nov 7 (Wed) 10:30-11:30, Park Hyatt Busan, Busan, Korea
Lecture: Oroboros for MIRACLE - the bioenergetic snapshot of mitochondrial fitness
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Oroboros Instruments is a member of COST Action CA15203 MitoEAGLE
Mitochondrial function in health and disease.png

Mitochondrial research and medicine at centre stage


The Oroboros O2k-FluoRespirometer provides the flexibility required to meet current demands in mitochondrial research and medicine:
  • Isolated mitochondria
  • Tissue homogenates
  • Permeabilized tissues
  • Permeabilized cells
  • Intac cells
  • Enzyme preparations




Bioenergetic snapshot.png

The bioenergetic snapshot of mitochondrial fitness


  • Fight the reproducibility crisis: shoot with accuracy
  • Analyze mitochondrial complexity: snapshot of mt-pathway and coupling control
  • Combine state and rate analysis:
  • O2 flux, O2 concentration (hypoxia and hyperoxia)
  • mitochondrial membrane potential
  • ATP production
  • ROS production
  • Ca2+
MitoEAGLE Proficiency ring test

The largest global mitochondrial network



O2k-FluoRespirometer

Oroboros exhibition

Oroboros and KR Seoul Scitech Korea are looking forward to welcoming you at the booth of Scitech Korea at ASMRM 2018.


Labels:






ORO, IOC, MitoGlobal, 2018, Next