MiPNet12.10 TIP2k-manual
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» Open Access-MiPNet12.10.pdf; Versions
OROBOROS (2014-03) Mitochondr Physiol Network
Abstract: Fasching M, Gradl L, Gradl P, Gnaiger E (2014) Titration-Injection microPump. TIP2k user manual. Mitochondr Physiol Network 12.10(06): 1-28.
The TIP2k provides unique advantages for applications which require highest accuracy of titration volumes and automatic control of complex titration-injection protocols. The TIP2k was specially developed for the Oxygraph-2k. The main modes of operation are automatic multi-step injections, continuous injections for steady-state respirometry, (both operated form the direct control mode), and feedback controlled operation.
Direct Control Mode: Operation mode for delivering a defined volume in a short pulse. A number of cycles, N, can be defined, and the duration of the interval from injection to injection determined, repeating the process N times. Several such steps can be programmed to run in sequence to give an entire TIP2k program.
Feedback Control Mode: Operation mode to reach and maintain a certain parameter (e.g. oxygen concentration) by automatic TIP injections.
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• Keywords: HRR, TIP2k
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, TIP2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
DatLab
Programming the TIP2k for "BG_Feedback"
In the TIP2k window the modus can be changed by clicking on either direct control or feedback control. In the feedback control several lines can be inserted, all of which take effect during the currently selected line of the main program. In the figure the implementation of program line 1 of the example set up is shown.
In the main window (Program line), blue highlighting shows that the first program line is selected. The first line in the feedback control window (upper right corner) sets the "start injection" value to 120 mM. The injection is only started when this condition is valid for two consecutive data points (DataN = 2). The interval is set to 200 seconds, which equals the maximum injection time when the maximum volume is set to 100 µl. These parameters are not important for the discussed purpose; however the interval has to be set to at least the (maximum) injection time calculated at the left side. Once an injection is started the program checks the following lines for "stop" instructions. If just one of these conditions is met, the injection will stop. In our example a stop condition is set at 100 µM.
