Difference between revisions of "MiPNet06.03 POS-calibration-SOP"

Latest revision as of 14:27, 27 July 2023

MiPNet06.03 POS-calibration-SOP

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

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Oroboros (2023-02-06) Mitochondr Physiol Network

Abstract: Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

• O2k-Network Lab: AT_Innsbruck_Oroboros

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

» Oxygen calibration - DatLab

Labels: MiParea: Respiration, Instruments;methods

HRR: Oxygraph-2k, O2k-Manual

DatLab, O2k-SOP, DL7, Oxygen solubility, DL8, MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS

@@ Line 1: / Line 1: @@
+{{Template:OROBOROS support page name}}
 {{Publication
-|title=Gnaiger E. Oxygen calibration and solubility in experimental media. Mitochondr. Physiol. Network 06.03.
+|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]]O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
-|info=[http://www.oroboros.at/index.php?oxygen-solubility  MiPNet06.03_O2-Calib-Solubility]
+|info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]]  » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
-|authors=Gnaiger E
+|authors=Oroboros
-|year=*
+|year=2023-02-06
-|journal=Mitochondr. Physiol. Network
+|journal=Mitochondr Physiol Network
-|abstract=[[High-resolution respirometry]] critically depends on accurate calibration of the [[polarographic oxygen sensor]]s which respond to [[partial oxygen pressure]].  Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to [[oxygen concentration]] requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The [[oxygen solubility]] of mitochondrial respiration medium [[MiR06]] relative to pure water (oxygen solubility factor, ''F''<sub>M</sub>) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; [[MiPNet14.13]]) at 37 °C and 30 °C.  At air saturation at standard barometric pressure (100 kPa) and 37 °C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 µM in MiR06.  A clean and ready-to-use Oxygraph chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
+|abstract=Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10.
-|mipnetlab=AT_Innsbruck_OROBOROS
+{{MiPNet pdf page linking to MitoPedia}}
-|articletype=Protocol; Manual, MiPNet-online Publication
+|mipnetlab=AT_Innsbruck_Oroboros
 }}
-{{Labeling
-|instruments=Oxygraph-2k, Protocol, MiPNet-Publication
-|additional=O2k-SOP
-|articletype=Protocol; Manual, MiPNet-online Publication
-}}
-==Info==
-MiPNet11.08 integrated into MiPNet06.03.
+== Keywords ==
+{{Template:Keywords: Oxygen signal}}
+::::» [[Oxygen calibration - DatLab]]
-==Contents==
-	O2k-Chamber: Cleaning and Storage
+{{Labeling
+|area=Respiration, Instruments;methods
-.1	Cleaning the Chamber
+|instruments=Oxygraph-2k, O2k-Manual
+|additional=DatLab, O2k-SOP, DL7, Oxygen solubility, DL8,
-.2	Storage in 70% Ethanol
+MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS
+}}
-	Short Two-Point Calibration
-	Equations for Oxygen Calibration
-.1	Oxygen Concentration and Recorded Signal
-.2	Oxygen Pressure and POS Current
-.3	Oxygen Concentration and Oxygen Pressure
-.4	Oxygen Signal and Background Oxygen Consumption
-	O2 Solubility and Concentration at Air Saturation
-.1	Oxygen Pressure and Concentration
-.2	Temperature Effect on Saturation O2 Conc.
-.3	Barometric Pressure Effect on Saturation O2 Conc.
-.4	The O2 Solubility Factor in Salt Solutions