Mastronicola 2011 IUBMB Life
| Mastronicola D, Giuffrè A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. IUBMB Life. 63(1):21-25. |
Mastronicola D, Giuffre A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) IUBMB Life
Abstract: Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O(2) -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O(2) -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O(2) to H(2) O rapidly (∼3 μM O(2) × min × 10(6) cells at 37 °C) and with high affinity (C(50) = 3.4 ± 0.7 μM O(2)). Following a short-term (minutes) exposure to H(2) O(2) ≥ 100 μM, the O(2) consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H(2) O(2) does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. • Keywords: o2-consumption, flavoprotein, detoxifying enzyme, oxidative stress, cell proteolysis, protozoan pathogen
Labels:
Organism: Bacteria"Bacteria" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
HRR: Oxygraph-2k
