Difference between revisions of "Mastronicola 2011 IUBMB Life"
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|year=2011 | |year=2011 | ||
|journal=IUBMB Life | |journal=IUBMB Life | ||
|abstract=Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O | |abstract=Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O<sub>2</sub> -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O<sub>2</sub> -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O<sub>2</sub> to H<sub>2</sub>O rapidly (∼3 μM O<sub>2</sub> × min × 10<sup>6</sup> cells at 37 °C) and with high affinity (C<sub>50</sub> = 3.4 ± 0.7 μM O<sub>2</sub>). Following a short-term (minutes) exposure to H<sub>2</sub>O<sub>2</sub> ≥ 100 μM, the O<sub>2</sub> consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H<sub>2</sub>O<sub>2</sub> does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. | ||
|keywords= | |keywords=Giardia intestinalis, oxygen consumption, flavoprotein, detoxifying enzyme, oxidative stress, cell proteolysis, protozoan pathogen | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|organism= | |injuries=RONS; Oxidative Stress | ||
|organism=Other Non-Mammal | |||
|preparations=Intact Cell; Cultured; Primary | |||
}} | }} | ||
Revision as of 13:23, 5 September 2011
| Mastronicola D, Giuffrè A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. IUBMB Life. 63(1):21-25. |
Mastronicola D, Giuffre A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) IUBMB Life
Abstract: Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O2 -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O2 -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O2 to H2O rapidly (∼3 μM O2 × min × 106 cells at 37 °C) and with high affinity (C50 = 3.4 ± 0.7 μM O2). Following a short-term (minutes) exposure to H2O2 ≥ 100 μM, the O2 consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H2O2 does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress. • Keywords: Giardia intestinalis, oxygen consumption, flavoprotein, detoxifying enzyme, oxidative stress, cell proteolysis, protozoan pathogen
Labels:
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Other Non-Mammal"Other Non-Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
