Difference between revisions of "Lin 2012 FASEB J"
(Created page with "{{Publication |title=Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD (2012) Low Intensity Exercise Attenuates Acute Lipid Loading-...") |
Beno Marija (talk | contribs) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD (2012) Low Intensity Exercise Attenuates Acute Lipid Loading-Induced Alterations in Mitochondrial Function in Rat Skeletal Muscle . FASEB J 1144.11. | |title=Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD (2012) Low Intensity Exercise Attenuates Acute Lipid Loading-Induced Alterations in Mitochondrial Function in Rat Skeletal Muscle . FASEB J 1144.11. | ||
|info=http://www.fasebj.org/cgi/content/meeting_abstract/26/1_MeetingAbstracts/1144.11 | |||
|authors=Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD | |authors=Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD | ||
|year=2012 | |year=2012 | ||
|journal=FASEB J | |journal=FASEB J | ||
|abstract=A single high-fat meal acutely increases skeletal muscle mitochondrial H2O2 emitting potential (mEH2O2), shifts the intracellular redox environment to a more oxidized state, and increases circulating markers of oxidative stress. Bioenergetically, this implies an acute lipid load may elevate the reducing pressure/membrane potential ({Delta}{Psi}m) within mitochondria and, conversely, that even a mild increase in energy expenditure may be sufficient to prevent these effects. To test this hypothesis, male Sprague-Dawley rats received an oral lipid gavage (20% intralipid, 45 Kcal/kg lean body mass) or water followed either by 2h of rest or 1h of rest plus 1h of low intensity treadmill exercise (15 m/min, 0% grade). Permeabilized fiber bundles were prepared from red gastrocnemius muscle for testing mitochondrial function. In rats receiving lipid, {Delta}{Psi}m and mEH2O2 were higher (P<0.05) and calcium retention capacity (mCa2+RC, an index of resistance to mitochondrial permeability transition) was lower under state IV and/or "clamped" ADP-stimulated state III conditions. All three effects were prevented when lipid gavage was followed by low-intensity exercise. Respiratory capacity was unaffected by any of the interventions. These findings provide evidence that mitochondrial {Delta}{Psi}m, mEH2O2, and mCa2+RC are acutely affected by nutritional overload in skeletal muscle, but can be prevented by low intensity exercise. NIH DK073488 | |abstract=A single high-fat meal acutely increases skeletal muscle mitochondrial H2O2 emitting potential (mEH2O2), shifts the intracellular redox environment to a more oxidized state, and increases circulating markers of oxidative stress. Bioenergetically, this implies an acute lipid load may elevate the reducing pressure/membrane potential ({Delta}{Psi}m) within mitochondria and, conversely, that even a mild increase in energy expenditure may be sufficient to prevent these effects. To test this hypothesis, male Sprague-Dawley rats received an oral lipid gavage (20% intralipid, 45 Kcal/kg lean body mass) or water followed either by 2h of rest or 1h of rest plus 1h of low intensity treadmill exercise (15 m/min, 0% grade). Permeabilized fiber bundles were prepared from red gastrocnemius muscle for testing mitochondrial function. In rats receiving lipid, {Delta}{Psi}m and mEH2O2 were higher (P<0.05) and calcium retention capacity (mCa2+RC, an index of resistance to mitochondrial permeability transition) was lower under state IV and/or "clamped" ADP-stimulated state III conditions. All three effects were prevented when lipid gavage was followed by low-intensity exercise. Respiratory capacity was unaffected by any of the interventions. These findings provide evidence that mitochondrial {Delta}{Psi}m, mEH2O2, and mCa2+RC are acutely affected by nutritional overload in skeletal muscle, but can be prevented by low intensity exercise. NIH DK073488 | ||
|mipnetlab=US NC Greenville Neufer PD | |mipnetlab=US NC Greenville Neufer PD, CA Toronto Perry CG | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Latest revision as of 16:56, 27 March 2018
| Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD (2012) Low Intensity Exercise Attenuates Acute Lipid Loading-Induced Alterations in Mitochondrial Function in Rat Skeletal Muscle . FASEB J 1144.11. |
» http://www.fasebj.org/cgi/content/meeting_abstract/26/1_MeetingAbstracts/1144.11
Lin CT, Kelsey H, Fisher-Wellman KH, Perry CG, Kozy R, Lark DS, Gilliam LA, Smith CD, Neufer PD (2012) FASEB J
Abstract: A single high-fat meal acutely increases skeletal muscle mitochondrial H2O2 emitting potential (mEH2O2), shifts the intracellular redox environment to a more oxidized state, and increases circulating markers of oxidative stress. Bioenergetically, this implies an acute lipid load may elevate the reducing pressure/membrane potential ({Delta}{Psi}m) within mitochondria and, conversely, that even a mild increase in energy expenditure may be sufficient to prevent these effects. To test this hypothesis, male Sprague-Dawley rats received an oral lipid gavage (20% intralipid, 45 Kcal/kg lean body mass) or water followed either by 2h of rest or 1h of rest plus 1h of low intensity treadmill exercise (15 m/min, 0% grade). Permeabilized fiber bundles were prepared from red gastrocnemius muscle for testing mitochondrial function. In rats receiving lipid, {Delta}{Psi}m and mEH2O2 were higher (P<0.05) and calcium retention capacity (mCa2+RC, an index of resistance to mitochondrial permeability transition) was lower under state IV and/or "clamped" ADP-stimulated state III conditions. All three effects were prevented when lipid gavage was followed by low-intensity exercise. Respiratory capacity was unaffected by any of the interventions. These findings provide evidence that mitochondrial {Delta}{Psi}m, mEH2O2, and mCa2+RC are acutely affected by nutritional overload in skeletal muscle, but can be prevented by low intensity exercise. NIH DK073488
• O2k-Network Lab: US NC Greenville Neufer PD, CA Toronto Perry CG
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Rat
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
HRR: Oxygraph-2k, TPP
