View source for Konczal 2015 Mol Biol Evol

{{Publication
|title=Konczal M, Babik W, Radwan J, Sadowska ET, Koteja P (2015) Initial molecular-level response to artificial selection for increased aerobic metabolism occurs primarily through changes in gene expression. Mol Biol Evol 32:1461-73.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25739734 PMID:25739734]
|authors=Konczal M, Babik W, Radwan J, Sadowska ET, Koteja P
|year=2015
|journal=Mol Biol Evol
|abstract=Experimental evolution combined with genome or transcriptome resequencing (Evolve and Resequence) represents a promising approach for advancing our understanding of the genetic basis of adaptation. Here, we applied this strategy to investigate the effect of selection on a complex trait in lines derived from a natural population of a small mammal. We analyzed the liver and heart transcriptomes of bank voles (Myodes [=Clethrionomys] glareolus) that had been selected for increased aerobic metabolism. The organs were sampled from 13th generation voles; at that point, the voles from four replicate selected lines had 48% higher maximum rates of oxygen consumption than those from four control lines. At the molecular level, the response to selection was primarily observed in gene expression: Over 300 genes were found to be differentially expressed between the selected and control lines and the transcriptome-wide pattern of expression distinguished selected lines from controls. No evidence for selection-driven changes of allele frequencies at coding sites was found: No single nucleotide polymorphism (SNP) changed frequency more than expected under drift alone and frequency changes aggregated over all SNPs did not separate selected and control lines. Nevertheless, among genes which showed highest differentiation in allele frequencies between selected and control lines we identified, using information about gene functions and the biology of the selected phenotype, plausible targets of selection; these genes, together with those identified in expression analysis, have been prioritized for further studies. Because our selection lines were derived from a natural population, the amount and the spectrum of variation available for selection probably closely approximated that typically found in populations of small mammals. Therefore, our results are relevant to the understanding of the molecular basis of complex adaptations occurring in natural vertebrate populations.
|keywords=RNA-Seq, Bank vole, Mammals, Maximum metabolic rate, Selection experiment, Standing genetic variation
}}
{{Labeling
|area=Respiration, Developmental biology
|organism=Other mammals
|tissues=Heart, Liver
}}