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IOC recommended reading

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Template:Mainpage OROBOROS


General reading

  • »Worksheet O2k-Basic: This worksheet contains questions on the O2k and HRR addressed to participants of O2k-Basic Workshops. Answers to these questions are elaborated during the O2k-Workshop.
  • Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access
    • The blue book: OXPHOS analysis; CI linked, CII linked, CI+II linked pathways, respiratory states, abbreviations
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Open Access
    • Introduction to high-resolution respirometry (HRR), SUIT protocols and flux control ratios.
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Open Access
    • Methodological introduction to HRR, with focus on Polarographic oxygen sensor and traditional oxygraphy
    • Dramatically different: High-resolution respirometry - the Oxygraph-2k
    • Calibration of polarographic oxygen sensors and oxygen concentration in respiration media at air saturation
    • From Oxygraph slopes to respiratory flux corrected for background effects
    • Phosphorylation or coupling control protocol (CCP) with intact cells
    • Titration steps of the CCP
    • Experimental example
    • Flux control ratios from the CCP
    • Intact cells, permeabilized cells and tissue, or isolated mitochondria?


Further reading

  • Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-1845. »Abstract
    • Respirometry with permeabilized fibres and isolated mitochondria
    • Convergent CI+II electron input and OXPHOS capacity
    • Tissue-OXPHOS capacity in human permeabilized muscle fibres and isolated mitochondria
    • Tissue-OXPHOS capacity and functional diversity
  • Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297. – A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen. »Abstract
    • Mitochondrial kinetics measured by high-resolution respirometry
    • Calibrations and corrections for response time and instrumental background
    • Steady-state injection respirometry
    • Mitochondrial respiratory control at low oxygen
    • Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
    • Effect of ADP and oxygen limitation on ADP/O2 flux ratios
    • The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
  • Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. »Abstract
    • Isolated mitochondria and intact cells
    • Nanomolar resolution of oxygen concentration
    • Effects of cell density and activity
    • Closed versus open systems
    • p50 in vivo
  • Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: 431-442. »Abstract
    • Isolated mitochondria and permeabilized muscle fibers, MiR05:
    • Optimization of mitochondrial cold storage • Mitochondrial respiration medium, MiR05 • Mitochondrial cold ischemia-reperfusion injury
  • Renner K, Amberger A, Konwalinka G, Kofler R, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642: 115-123. »Abstract
    • Intact cells
    • Cytochrome c oxidase
    • Cytochrome c test
    • Respiration per million cells, per citrate synthase, per mg protein, or per cytochrome c oxidase activity.