Difference between revisions of "High signal at zero oxygen"

Revision as of 15:31, 17 January 2022

High signal at zero oxygen

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

A high signal at zero oxygen may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem. For O2k series A-C, please start with the POS-Connector already connected to the main unit and proceed with step 5.

Abbreviation: R0

Reference: Zero calibration, OroboPOS-Connector

High zero current test

1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.

2. Switch on the O2k and start DatLab.

3. Record the signal for 10 min.

4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".

5. Record the signal for 10 min.

6. Remove the membrane from the POS and wash the cathode and anode with distilled water.

7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".

8. Record the signal for 10 min.

9. Send us the file.

References:

[1] Cleaning the electrical connections

[2] MiPNet19.18B POS-service

[3] MiPNet06.03 POS-calibration-SOP

[4] MiPNet14.06 Instrumental O2 background

Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O₂ raw signal [V] was recorded for 10 min. The marks were set to the O₂ raw signal [V] to evaluate the zero current.

Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry

@@ Line 1: / Line 1: @@
-{{MitoPedia
+{{Technical support}}
-|description='A ''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem.
+__TOC__
+{{MitoPedia without banner
+|abbr=''R''0
+|description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem. For O2k series A-C, please start with the POS-Connector already connected to the main unit and proceed with step 5.
+|info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]]
 }}
-{{MitoPedia concepts}}
+== High zero current test==
-{{MitoPedia methods}}
+::::1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
+::::2. Switch on the O2k and start DatLab.
+::::3. Record the signal for 10 min.
+::::4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
+::::5. Record the signal for 10 min.
+::::6. Remove the membrane from the POS and wash the cathode and anode with distilled water.
+::::7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
+::::8. Record the signal for 10 min.
+::::9. Send us the file.
+:::::References:
+::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections]
+::::::[2] [[MiPNet19.18B POS-service]]
+::::::[3] [[MiPNet06.03 POS-calibration-SOP]]
+::::::[4] [[MiPNet14.06 Instrumental O2 background]]
+::: '''Example:''' First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current.
+::::[[File:High zero current good example.png|700px]]
+::::[[File:High zero current test bad exmaple.png|700px]]
+::::'''Figure 1.''' High zero current test.  '''Upper panel:''' Zero current is in the range of ±0.00099 V. '''Lower panel:''' Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.
+== Keywords ==
+{{Template:Keywords: Oxygen signal}}
 {{MitoPedia O2k and high-resolution respirometry
 |mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry
 }}
-{{MitoPedia topics}}