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Difference between revisions of "High signal at zero oxygen"

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(Created page with "{{MitoPedia |description='A ''high signal at zero oxygen''' may be observed during zero calibration. The following instructions show how to further localize and possibly s...")
 
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{{MitoPedia
{{Technical support}}
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|description='A ''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem.
|description='A ''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem.
}}
}}
{{MitoPedia concepts}}
== High zero current ==
{{MitoPedia methods}}
:::: In order to localize the problem, the following test should be performed:
 
:::[[File:High zero current test 2.png|1000px]]
::::*Note: Before sending us the O2k or any spare components, please contact us via our O2k-Open Support system: [[O2k-Open Support]]
:::::References:
::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections]
::::::[2] [[MiPNet19.18B POS-service]]
::::::[3] [[MiPNet06.03 POS-calibration-SOP]]
::::::[4] [[MiPNet14.06 Instrumental O2 background]]
 
::: '''Example:'''
::::[[File:High zero current good example.png|600px]]
::::[[File:High zero current test bad exmaple.png|600px]]
::::'''Figure 1.''' High zero current test.  Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS have electrical problems. First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current.
 
 
== Keywords ==
{{Template:Keywords: Oxygen signal}}
 
 
{{MitoPedia O2k and high-resolution respirometry
{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry
}}
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{{MitoPedia topics}}

Revision as of 16:56, 29 October 2020


                  


O2k-Open Support

High signal at zero oxygen



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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

'A high signal at zero oxygen' may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem.

High zero current

In order to localize the problem, the following test should be performed:
High zero current test 2.png
  • Note: Before sending us the O2k or any spare components, please contact us via our O2k-Open Support system: O2k-Open Support
References:
[1] Cleaning the electrical connections
[2] MiPNet19.18B POS-service
[3] MiPNet06.03 POS-calibration-SOP
[4] MiPNet14.06 Instrumental O2 background
Example:
High zero current good example.png
High zero current test bad exmaple.png
Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS have electrical problems. First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the zero current.


Keywords


Questions.jpg


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MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry