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Difference between revisions of "Gnaiger 2001 Respir Physiol"

From Bioblast
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|mipnetlab=AT_Innsbruck_GnaigerE
|mipnetlab=AT_Innsbruck_GnaigerE
|abstract=Oxygen limitation is generally considered as impairment of mitochondrial respiration under hypoxia and ischemia. Low intracellular oxygen levels under normoxia, however, imply mild oxygen limitation, provide protection from oxidative stress, and result from economical strategies for oxygen transport through the respiratory cascade to cytochrome c oxidase. Both perspectives relate to the critical oxygen pressure which inhibits mitochondrial respiration. Based on methodological considerations of oxygen kinetics and a presentation of [[high-resolution respirometry]], mitochondrial oxygen affinities (1/P50) are reviewed with particular emphasis on the turnover effect under control of ADP, which increases the P50 in active states. ADP/O2 flux
|abstract=Oxygen limitation is generally considered as impairment of mitochondrial respiration under hypoxia and ischemia. Low intracellular oxygen levels under normoxia, however, imply mild oxygen limitation, provide protection from oxidative stress, and result from economical strategies for oxygen transport through the respiratory cascade to cytochrome c oxidase. Both perspectives relate to the critical oxygen pressure which inhibits mitochondrial respiration. Based on methodological considerations of oxygen kinetics and a presentation of [[high-resolution respirometry]], mitochondrial oxygen affinities (1/P50) are reviewed with particular emphasis on the turnover effect under control of ADP, which increases the P50 in active states. ADP/O2 flux
ratios are high even under severe oxygen limitation, as demonstrated by calorespirometry. Oxygen limitation reduces the uncoupled respiration observed under control by ADP, as shown by relationships derived between ADP/O2 flux
ratios are high even under severe oxygen limitation, as demonstrated by calorespirometry. Oxygen limitation reduces the uncoupled respiration observed under control by ADP, as shown by relationships derived between ADP/O2 flux ratios, respiratory control ratios, and ADP kinetics. Bioenergetics at low oxygen versus oxidative stress must be considered in the context of limitation of maximum aerobic activity, ischemia-reperfusion injury, mitochondrial signalling to apoptosis, and mitochondrial theories of ageing.
ratios, respiratory control ratios, and ADP kinetics. Bioenergetics at low oxygen versus oxidative stress must be considered in the context of limitation of maximum aerobic activity, ischemia-reperfusion injury, mitochondrial
signalling to apoptosis, and mitochondrial theories of ageing.
|keywords=Energy: Oxidative phosphorylation, Adenosine diphosphate kinetics, Adenosine diphosphate/O2 ratio; Hypoxia: Mitochondrial O2 kinetics; Mammals: Rat; Membrane permeability;Mitochondria: heart, liver
|keywords=Energy: Oxidative phosphorylation, Adenosine diphosphate kinetics, Adenosine diphosphate/O2 ratio; Hypoxia: Mitochondrial O2 kinetics; Mammals: Rat; Membrane permeability;Mitochondria: heart, liver
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11718759 PMID: 11718759]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11718759 PMID: 11718759]

Revision as of 16:16, 13 September 2010

Publications in the MiPMap
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir. Physiol. 128: 277-297.

Β» PMID: 11718759

Gnaiger E (2001) Respir. Physiol.

Abstract: Oxygen limitation is generally considered as impairment of mitochondrial respiration under hypoxia and ischemia. Low intracellular oxygen levels under normoxia, however, imply mild oxygen limitation, provide protection from oxidative stress, and result from economical strategies for oxygen transport through the respiratory cascade to cytochrome c oxidase. Both perspectives relate to the critical oxygen pressure which inhibits mitochondrial respiration. Based on methodological considerations of oxygen kinetics and a presentation of high-resolution respirometry, mitochondrial oxygen affinities (1/P50) are reviewed with particular emphasis on the turnover effect under control of ADP, which increases the P50 in active states. ADP/O2 flux ratios are high even under severe oxygen limitation, as demonstrated by calorespirometry. Oxygen limitation reduces the uncoupled respiration observed under control by ADP, as shown by relationships derived between ADP/O2 flux ratios, respiratory control ratios, and ADP kinetics. Bioenergetics at low oxygen versus oxidative stress must be considered in the context of limitation of maximum aerobic activity, ischemia-reperfusion injury, mitochondrial signalling to apoptosis, and mitochondrial theories of ageing. β€’ Keywords: Energy: Oxidative phosphorylation, Adenosine diphosphate kinetics, Adenosine diphosphate/O2 ratio; Hypoxia: Mitochondrial O2 kinetics; Mammals: Rat; Membrane permeability;Mitochondria: heart, liver

β€’ O2k-Network Lab: AT_Innsbruck_GnaigerE


Labels:

Stress:Hypoxia  Organism: Human, Rat  Tissue;cell: Cardiac Muscle"Cardiac Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property., Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property., Endothelial; Epithelial; Mesothelial Cell"Endothelial; Epithelial; Mesothelial Cell" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k, TIP2k, CaloRespirometry; Twin-Flow"CaloRespirometry; Twin-Flow" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.