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Difference between revisions of "Garcia-Neto 2017 PLOS ONE"

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|pathways=N, S, CIV, ROX
|pathways=N, S, CIV, ROX
|instruments=Oxygraph-2k
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Revision as of 13:16, 13 November 2017

Publications in the MiPMap
Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, Alberto Luévano-Martínez L (2017) High osmolarity environments activate the mitochondrial alternative oxidase in Debaryomyces Hansenii. PLOS ONE 12:e0169621.

» PMID: 28060946 Open Access

Garcia-Neto W, Cabrera-Orefice A, Uribe-Carvajal S, Kowaltowski AJ, Luevano-Martinez AL (2017) PLOS ONE

Abstract: The oleaginous yeast Debaryomyces hansenii is a good model to understand molecular mechanisms involved in halotolerance because of its impressive ability to survive under a wide range of salt concentrations. Several cellular adaptations are implicated in this response, including the presence of a cyanide-insensitive ubiquinol oxidase (Aox). This protein, which is present in several taxonomical orders, has been related to different stress responses. However, little is known about its role in mitochondria during transitions from low to high saline environments. In this report, we analyze the effects of Aox in shifts from low to high salt concentrations in the culture media. At early stages of a salt insult, we observed that this protein prevents the overflow of electrons on the mitochondrial respiratory chain, thus, decreasing the production of reactive oxygen species. Interestingly, in the presence of high osmolite concentrations, Aox activity is able to sustain a stable membrane potential when coupled to complex I, despite a compromised cytochrome pathway. Taken together, our results suggest that under high osmolarity conditions Aox plays a critical role regulating mitochondrial physiology.

Bioblast editor: Kandolf G O2k-Network Lab: BR Sao Paulo Kowaltowski AJ


Labels: MiParea: Respiration, Comparative MiP;environmental MiP 


Organism: Fungi 

Preparation: Isolated mitochondria 


Coupling state: ET  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k 

Labels