Difference between revisions of "File:Cell respiration stocks.png"
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:::: '''Fig. 1.5.''' Partitioning of a sample of suspended cells of suspension volume ''V''<sub>s</sub> [mL]. (1) ''v''<sub>s1</sub> for cell count ''N''<sub>ce</sub> [Mx = 10<sup>6</sup> x]; (2) cell-count concentration ''C''<sub>ce</sub> [Mx∙mL<sup>-1</sup>]; (3) ''v''<sub>s2</sub> for cell wet mass ''m''<sub>ce</sub> [mg] or other markers of cell size and mt-markers; (4) mass per elementary cell ''M''<sub>''U''<sub>ce</sub></sub> [mg∙Mx<sup>-1</sup> = ng∙x<sup>-1</sup>]; (5) ''v''<sub>s3</sub> or ''V''<sub>stock</sub> for the respirometric stock of suspended cells; and (6) ''v''<sub>stock''i''</sub> subsamples for technical repeats of respirometric chambers. Typical numerical values are given for '''(a)''' human umbilical vein endothelial cells HUVEV or fibroblasts, and '''(b)''' cryopreserved HEK 293 cells. The targets are a sample-cell suspension with a cell-count concentration of 10 to 40 Mx∙mL<sup>-1</sup>; a final cell-count concentration of 1 Mx∙mL<sup>-1</sup> in the respirometer chamber, which may be reduced to 0.5 Mx∙mL<sup>-1</sup>; a subsample titration volume of ''v''<sub>stock''i''</sub> = 0.1 mL to 0.5 mL into the 0.5- or 2.0-mL experimental chamber; and a wet cell mass of about 1 mg for gravimetric measurement or other assays. For HUVEC with a radius of 6.8 µm of the spherical suspended cells, the elementary cell volume ''V''<sub>''U''<sub>ce</sub></sub> is 0.0013 nL∙x<sup>-1</sup> (1300 fL∙x<sup>-1</sup>; Gnaiger 2003), and at a cell density of 1.06 µg∙nL<sup>-1</sup>, the elementary cell mass ''M''<sub>''U''<sub>ce</sub></sub> is 1.38 mg∙Mx<sup>-1</sup> (equivalent to ng∙x<sup>-1</sup>). | |||
Revision as of 14:42, 3 September 2020
- Fig. 1.5. Partitioning of a sample of suspended cells of suspension volume Vs [mL]. (1) vs1 for cell count Nce [Mx = 106 x]; (2) cell-count concentration Cce [Mx∙mL-1]; (3) vs2 for cell wet mass mce [mg] or other markers of cell size and mt-markers; (4) mass per elementary cell MUce [mg∙Mx-1 = ng∙x-1]; (5) vs3 or Vstock for the respirometric stock of suspended cells; and (6) vstocki subsamples for technical repeats of respirometric chambers. Typical numerical values are given for (a) human umbilical vein endothelial cells HUVEV or fibroblasts, and (b) cryopreserved HEK 293 cells. The targets are a sample-cell suspension with a cell-count concentration of 10 to 40 Mx∙mL-1; a final cell-count concentration of 1 Mx∙mL-1 in the respirometer chamber, which may be reduced to 0.5 Mx∙mL-1; a subsample titration volume of vstocki = 0.1 mL to 0.5 mL into the 0.5- or 2.0-mL experimental chamber; and a wet cell mass of about 1 mg for gravimetric measurement or other assays. For HUVEC with a radius of 6.8 µm of the spherical suspended cells, the elementary cell volume VUce is 0.0013 nL∙x-1 (1300 fL∙x-1; Gnaiger 2003), and at a cell density of 1.06 µg∙nL-1, the elementary cell mass MUce is 1.38 mg∙Mx-1 (equivalent to ng∙x-1).
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