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Difference between revisions of "ET capacity"

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Respiratory electron transfer system capacity of mitochondria in the experimentally induced state of [[non-coupled respiration]] (fully uncoupled; state ''E''), obtained in intact cells or mitochondrial preparations with defined substrates, by titration of an established [[uncoupler]] up to optimum concentration at maximum flux.Β  Non-coupled respiration yields an estimate of ETS capacity.Β  In this state ''[[E]]'', the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and for measurement of the mt-membrane potential.
{{MitoPedia
==References==
|description='''Respiratory [[electron transfer system]] capacity''' of mitochondria in the experimentally induced state of [[non-coupled respiration]] (fully uncoupled; state ''E''), obtained in intact cells or mitochondrial preparations with defined substrates, by titration of an established [[uncoupler]] up to optimum concentration at maximum flux.Β  Non-coupled respiration yields an estimate of ETS capacity.Β  In this state ''[[E]]'', the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and for measurement of the mt-membrane potential.
*Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int. J. Biochem. Cell Biol. 41: 1837–1845. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCH-4W04KR1-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a6456620daa0d8cca022b5f934f2cf30 ''- link'']
*Gnaiger E (2010) MitoPathways: Respiratory states and flux control ratios. Mitochondr. Physiol. Network 12.15: 1-5. [http://www.oroboros.at/index.php?respiratorystates ''- link'']


{{#set:keyword=Respiration}}
Β 
{{MitoPedia}}
|info=[[MiPNet12.15]]; [[Gnaiger_2009_IJCBC]]
|type=Respiration
}}
{{Labeling
|discipline=Mitochondrial Physiology
}}

Revision as of 11:41, 14 September 2010


high-resolution terminology - matching measurements at high-resolution


ET capacity

Description

Respiratory electron transfer system capacity of mitochondria in the experimentally induced state of non-coupled respiration (fully uncoupled; state E), obtained in intact cells or mitochondrial preparations with defined substrates, by titration of an established uncoupler up to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of ETS capacity. In this state E, the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and for measurement of the mt-membrane potential.


Reference: MiPNet12.15; Gnaiger_2009_IJCBC


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