Difference between revisions of "Chicco 2022 Abstract Bioblast"
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{{Abstract | {{Abstract | ||
|title=[[File:Chicco Headshot.jpg|left|100px|Chicco Adam]] <u>Chicco Adam</u>, Zilhaver PT, Whitcomb LA, Fresa KJ, Izon CS, Gonzalez-Franquesa A, Izon CS, Dometita C, Irving BA, Garcia-Roves PM (2022) Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols. Bioblast 2022: BEC Inaugural Conference. <br>[[Chicco 2022 MitoFit|»''MitoFit Preprint''«]] | |title=[[File:Chicco Headshot.jpg|left|100px|Chicco Adam]] <u>Chicco Adam</u>, Zilhaver PT, Whitcomb LA, Fresa KJ, Izon CS, Gonzalez-Franquesa A, Izon CS, Dometita C, Irving BA, Garcia-Roves PM (2022) Resolving the Rotenone Paradox: elucidating the complexity of multi-substrate respirometry protocols. Bioblast 2022: BEC Inaugural Conference. <br>[[Chicco 2022 MitoFit|»''MitoFit Preprint''«]] | ||
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|year=2022 | |year=2022 | ||
|event=[[Bioblast 2022]] | |event=[[Bioblast 2022]] | ||
|abstract= | |abstract=Multi-substrate respirometry protocols are frequently used to resolve the relative contributions of NADH-producing (N-pathway or CI-linked) substrates and succinate (S-pathway or CII-linked substrate) to mitochondrial oxygen consumption rate (''J''<sub>O<sub>2</sub></sub>). Similarly, rotenone (a selective CI inhibitor) is utilized in the presence of NS-substrates to deduce the contribution of N-pathway flux to the total (NS-pathway) ''J''<sub>O<sub>2</sub></sub>. However, under S- and some NS-pathway states, rotenone elicits a paradoxical increase in ''J''<sub>O<sub>2</sub></sub>, revealing a complex interaction of N- and S-pathway substrate oxidation on ''J''<sub>O<sub>2</sub></sub> ''in vitro''. Herein, we demonstrate inhibitory effects of >1 mM malate or malonate (a CII inhibitor) on ''J''<sub>O<sub>2</sub></sub> supported by pyruvate and/or glutamate, suggesting that endogenous succinate oxidation interacts with malate concentration to potently regulate ''J''<sub>O<sub>2</sub></sub> supported by N-pathway substrates in a tissue-specific manner. Potential mechanisms are discussed to stimulate further experimentation aimed at elucidating the biological bases for variations in NS-pathway flux in multi-substrate respirometry protocols. | ||
Multi-substrate respirometry protocols are frequently used to resolve the relative contributions of NADH-producing (N-pathway or CI-linked) substrates and succinate (S-pathway or CII-linked substrate) to mitochondrial oxygen consumption rate (''J<sub> | |||
|keywords=Mitochondrial respiration, electron transport chain, succinate, glutamate, oxidative phosphorylation, high-resolution respirometry | |keywords=Mitochondrial respiration, electron transport chain, succinate, glutamate, oxidative phosphorylation, high-resolution respirometry | ||
|mipnetlab=US CO Fort Collins Chicco AJ | |mipnetlab=US CO Fort Collins Chicco AJ | ||
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== Affiliations == | == Affiliations == | ||
:::: Chicco AJ | :::: Chicco AJ<sup>1</sup>, Zilhaver PT<sup>1</sup>, Whitcomb LA<sup>1</sup>, Fresa KJ<sup>1</sup>, Izon CS<sup>1</sup>, Gonzalez-Franquesa A<sup>2</sup>, Dometita C<sup>3</sup>, Irving BA<sup>3,4</sup>, Garcia-Roves PM<sup>5</sup> | ||
::::# Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA - [email protected] | |||
::::# NovoNordisk, Copenhagen, Denmark | |||
::::# Geisinger Obesity Institute, Danville, Pennsylvania, USA | |||
::::# Department of Kinesiology, Louisiana State University, Baton Rouge, Louisiana, USA | |||
::::# Department of Physiological Sciences, University of Barcelona, Barcelona, Spain | |||
== Help == | == Help == | ||
Revision as of 14:03, 22 May 2022
 »MitoFit Preprint« |
Link: Bioblast 2022: BEC Inaugural Conference
Chicco Adam J, Zilhaver Philip T, Whitcomb Luke A, Fresa Kyle J, Izon Cheyanne S, Gonzalez-Franquesa Alba, Dometita Crystal, Irving Brian A, Garcia-Roves Pablo Miguel (2022)
Event: Bioblast 2022
Multi-substrate respirometry protocols are frequently used to resolve the relative contributions of NADH-producing (N-pathway or CI-linked) substrates and succinate (S-pathway or CII-linked substrate) to mitochondrial oxygen consumption rate (JO2). Similarly, rotenone (a selective CI inhibitor) is utilized in the presence of NS-substrates to deduce the contribution of N-pathway flux to the total (NS-pathway) JO2. However, under S- and some NS-pathway states, rotenone elicits a paradoxical increase in JO2, revealing a complex interaction of N- and S-pathway substrate oxidation on JO2 in vitro. Herein, we demonstrate inhibitory effects of >1 mM malate or malonate (a CII inhibitor) on JO2 supported by pyruvate and/or glutamate, suggesting that endogenous succinate oxidation interacts with malate concentration to potently regulate JO2 supported by N-pathway substrates in a tissue-specific manner. Potential mechanisms are discussed to stimulate further experimentation aimed at elucidating the biological bases for variations in NS-pathway flux in multi-substrate respirometry protocols.
• Keywords: Mitochondrial respiration, electron transport chain, succinate, glutamate, oxidative phosphorylation, high-resolution respirometry
• O2k-Network Lab: US CO Fort Collins Chicco AJ
Affiliations
- Chicco AJ1, Zilhaver PT1, Whitcomb LA1, Fresa KJ1, Izon CS1, Gonzalez-Franquesa A2, Dometita C3, Irving BA3,4, Garcia-Roves PM5
- Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA - [email protected]
- NovoNordisk, Copenhagen, Denmark
- Geisinger Obesity Institute, Danville, Pennsylvania, USA
- Department of Kinesiology, Louisiana State University, Baton Rouge, Louisiana, USA
- Department of Physiological Sciences, University of Barcelona, Barcelona, Spain
Help
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