Difference between revisions of "Beryllium sulfate"
From Bioblast
| Line 29: | Line 29: | ||
== References == | == References == | ||
::::* [ | ::::* [[Chinopoulos 2014 Methods Enzymol]] | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
Latest revision as of 12:57, 26 April 2024
- high-resolution terminology - matching measurements at high-resolution
Beryllium sulfate
Description
Beryllium sulfate is used in combination with sodium fluoride to form beryllium trifluoride (BeF3−), to inhibit the ATP synthase if it is exposed by disruption of the mitochondrial membranes.
Abbreviation: BeS
Application in HRR
- BeS: (Beryllium sulfate; BeSO4), store at 4 °C, M = 177.14 g·mol-1
- Preparation of 200 mM stock solution (dissolved in H2O)
- Weigh 354.3 mg.
- Dissolve in 10 mL distilled H2O under a fume hood.
- Divide in aliquots.
- Store at +4 °C;
- Preparation of 200 mM stock solution (dissolved in H2O)
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 µL using a 10 µL syringe (2 mL O2k-chamber).
- Final concentration: 200 µM.
References
MitoPedia topics: Inhibitor
