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Difference between revisions of "Amytal"

From Bioblast
(replaced FW by M and added units g*mol-1)
(replaced 99.9 % ethanol by ethanol abs.)
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::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>
::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span>


::::'''Preparation of 200 mM stock solution''' (dissolved in 99.9% ethanol):
::::'''Preparation of 200 mM stock solution''' (dissolved in ethanol abs.):


::::# Weigh 49.65 mg of amytal.
::::# Weigh 49.65 mg of amytal.

Latest revision as of 11:53, 22 June 2021


high-resolution terminology - matching measurements at high-resolution


Amytal

Description

Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.

Abbreviation: Amy


Application in HRR

Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; M = 248.25 gยทmol-1. The product is withdrawn from Sigma but some residual amounts are still available.
Caution: Light sensitive and toxic!
Preparation of 200 mM stock solution (dissolved in ethanol abs.):
  1. Weigh 49.65 mg of amytal.
  2. Dissolve in 10 mL 50% ethanol.
  3. Divide into 500 ยตL aliquots (use dark glass vials - light sensitive).
  4. Store at -20 ยฐC.



MitoPedia topics: Inhibitor