- high-resolution terminology - matching measurements at high-resolution
Amplex UltraRed
Description
Amplex red (AmR) belongs to the extrinsic fluorophores and makes use of the fact that ROS generate hydrogen peroxide (H2O2) and it is the H2O2 level that Amplex red is used to measure. In the presence of horseradish peroxidase, Amplex red reacts with H2O2 to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H2O2 concentration.
Abbreviation: AmR
Reference: Mishin 2010 Free Radical Biol Med, Towne 2004 Anal Biochem, Hickey 2012 J Comp Physiol B
MitoPedia methods:
Fluorometry
This method can be used with the [O2k-Fluorescence LED2-Module]][1]. In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the black PEEK stopper[2].
References
