Difference between revisions of "Amplex UltraRed"
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This method can be used with the [[O2k-Fluorescence LED2-Module]]<ref>[[Hickey 2012 J Comp Physiol B]]</ref>. In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the [[Stopper\black PEEK\conical Shaft\central+side 2.5 mm Port|black PEEK stopper]]<ref> [[Anderson 2011 Am J Physiol Heart Circ Physiol]])</ref>. | This method can be used with the [[O2k-Fluorescence LED2-Module]]<ref>[[Hickey 2012 J Comp Physiol B]]</ref>. A template for H2O2 calibration is available for download [http://www.oroboros.at/index.php?id=o2k-fluorescence @OROBOROS] In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the [[Stopper\black PEEK\conical Shaft\central+side 2.5 mm Port|black PEEK stopper]]<ref> [[Anderson 2011 Am J Physiol Heart Circ Physiol]])</ref>. | ||
Please see also the [[Talk:Amplex red|Discussion page]]! | Please see also the [[Talk:Amplex red|Discussion page]]! | ||
Revision as of 13:47, 21 December 2012
- high-resolution terminology - matching measurements at high-resolution
Amplex UltraRed
Description
Amplex red (AmR) belongs to the extrinsic fluorophores and makes use of the fact that ROS generate hydrogen peroxide (H2O2) and it is the H2O2 level that Amplex red is used to measure. In the presence of horseradish peroxidase, Amplex red reacts with H2O2 to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H2O2 concentration.
Abbreviation: AmR
Reference: Mishin 2010 Free Radical Biol Med, Towne 2004 Anal Biochem, Hickey 2012 J Comp Physiol B, Fasching 2011 Abstract Berlin
MitoPedia methods:
Fluorometry
This method can be used with the O2k-Fluorescence LED2-Module[1]. A template for H2O2 calibration is available for download @OROBOROS In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the black PEEK stopper[2].
Please see also the Discussion page!
References
