Difference between revisions of "Amplex UltraRed"
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|abbr=AmR | |abbr=AmR | ||
|description='''Amplex red''' (AmR) belongs to the [[extrinsic fluorophores]] and makes use of the fact that [[ROS]] generate [[hydrogen peroxide]] (H<sub>2</sub>O<sub>2</sub>) and it is the H<sub>2</sub>O<sub>2</sub> level that Amplex red is used to measure. In the presence of [[horseradish peroxidase]], Amplex red reacts with H<sub>2</sub>O<sub>2</sub> to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H<sub>2</sub>O<sub>2</sub> concentration. | |description='''Amplex red''' (AmR) belongs to the [[extrinsic fluorophores]] and makes use of the fact that [[ROS]] generate [[Measuring hydrogen peroxide|hydrogen peroxide]] (H<sub>2</sub>O<sub>2</sub>) and it is the H<sub>2</sub>O<sub>2</sub> level that Amplex red is used to measure. In the presence of [[horseradish peroxidase]], Amplex red reacts with H<sub>2</sub>O<sub>2</sub> to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H<sub>2</sub>O<sub>2</sub> concentration. | ||
|info=[[Mishin 2010 Free Radical Biol Med]], [[Towne 2004 Anal Biochem]], [[Hickey 2012 J Comp Physiol B]], [[Fasching 2011 Abstract Berlin]] | |info=[[Mishin 2010 Free Radical Biol Med]], [[Towne 2004 Anal Biochem]], [[Hickey 2012 J Comp Physiol B]], [[Fasching 2011 Abstract Berlin]] | ||
}} | }} | ||
Revision as of 15:06, 20 June 2012
- high-resolution terminology - matching measurements at high-resolution
Amplex UltraRed
Description
Amplex red (AmR) belongs to the extrinsic fluorophores and makes use of the fact that ROS generate hydrogen peroxide (H2O2) and it is the H2O2 level that Amplex red is used to measure. In the presence of horseradish peroxidase, Amplex red reacts with H2O2 to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H2O2 concentration.
Abbreviation: AmR
Reference: Mishin 2010 Free Radical Biol Med, Towne 2004 Anal Biochem, Hickey 2012 J Comp Physiol B, Fasching 2011 Abstract Berlin
MitoPedia methods:
Fluorometry
This method can be used with the O2k-Fluorescence LED2-Module[1]. In addition, the Oxygraph-2k has been coupled to full fluorescence spectrophotometers for this purpose, with a light guide inserted through the black PEEK stopper[2].
Please see also the Discussion page!
References
