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Difference between revisions of "Air calibration"

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Air calibration is together with zero calibration one of the two steps of the [[POS calibration]], see [[http://www.oroboros.at/index.php?id=o2k-o2calibration MiPNet12.08]]. It is done at [[open chamber]] after zero( +/- 1 pmol (s ml)) has been achieved. Unlike [[zero calibration]] but like the [[stirrer test]], air calibration should be done before each experiment (and does not take any extra time).
{{MitoPedia
See [Raw signal]] for the signal to expect during Air calibration.
|abbr=R1
|description='''Air calibration''' of an oxygen sensor (polarographic oxygen sensor) is performed routinely on any day before starting a respirometric experiment. The volume fraction of oxygen in dry air is constant. An aqueous solution in equilibrium with air has the same partial pressure as that in water vapour saturated air. The water vapour is a function of temperature only. The partial oxygen pressure in aqueous solution in equilibrium with air is, therefore, a function of total barometric pressure and temperature. Bubbling an aqueous solution with air generates deviations from barometric pressure within small gas bubbles and is, therefore, not recommended. To equilibrate an aqueous solution ata known partial pressure of oxygen [kPa], the aqueous solution is stirred rigorously in a chamber enclosing air at constant temperature. The concentration of oxygen, ''c''<sub>O2</sub> [µM], is obtained at any partial pressure by multiplying the partial pressure by the oxygen solubility, ''S''<sub>O2</sub> [µM/kPa]. ''S''<sub>O2</sub> is a function of temperature and composition of the salt solution, and is thus a function of the experimental medium. The [[Oxygen_solubility_factor|solubility factor]] of the medium, ''F''<sub>M</sub>, expresses the oxygen solubility relative to pure water at any experimental temperature. ''F''<sub>M</sub> is 0.89 in serum (37 °C) and 0.92 in [[MiR06]] or [[MiR05]] (30 °C and 37 °C).
|info=[[MiPNet06.03 POS-calibration-SOP]], [[Oxygen calibration - DatLab]], [[Oxygen sensor test]]
}}


{{#set:Technical service= POS calibration}}
{{Template:Keywords: Oxygen signal}}


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{{MitoPedia methods
 
|mitopedia method=Respirometry
{{Technical service}}
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{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=DatLab, Oroboros QM
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{{MitoPedia topics
|mitopedia topic=Medium
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Latest revision as of 10:01, 5 October 2020


high-resolution terminology - matching measurements at high-resolution


Air calibration

Description

Air calibration of an oxygen sensor (polarographic oxygen sensor) is performed routinely on any day before starting a respirometric experiment. The volume fraction of oxygen in dry air is constant. An aqueous solution in equilibrium with air has the same partial pressure as that in water vapour saturated air. The water vapour is a function of temperature only. The partial oxygen pressure in aqueous solution in equilibrium with air is, therefore, a function of total barometric pressure and temperature. Bubbling an aqueous solution with air generates deviations from barometric pressure within small gas bubbles and is, therefore, not recommended. To equilibrate an aqueous solution ata known partial pressure of oxygen [kPa], the aqueous solution is stirred rigorously in a chamber enclosing air at constant temperature. The concentration of oxygen, cO2 [µM], is obtained at any partial pressure by multiplying the partial pressure by the oxygen solubility, SO2 [µM/kPa]. SO2 is a function of temperature and composition of the salt solution, and is thus a function of the experimental medium. The solubility factor of the medium, FM, expresses the oxygen solubility relative to pure water at any experimental temperature. FM is 0.89 in serum (37 °C) and 0.92 in MiR06 or MiR05 (30 °C and 37 °C).

Abbreviation: R1

Reference: MiPNet06.03 POS-calibration-SOP, Oxygen calibration - DatLab, Oxygen sensor test



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MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: DatLab, Oroboros QM 


MitoPedia topics: Media for respirometry