Vijgen 2013 Surg Obes Relat Dis
|Vijgen GH, Bouvy ND, Hoeks J, Wijers S, Schrauwen P, van Marken Lichtenbelt WD (2013) Impaired skeletal muscle mitochondrial function in morbidly obese patients is normalized one year after bariatric surgery. Surg Obes Relat Dis 9:936-41.|
Abstract: BACKGROUND: Obesity and type 2 diabetes are associated with impaired skeletal muscle mitochondrial metabolism. As an intrinsic characteristic of an individual, skeletal muscle mitochondrial dysfunction could be a risk factor for weight gain and obesity-associated co-morbidities, such as type 2 diabetes. On the other hand, impaired skeletal muscle metabolism could be a consequence of obesity. We hypothesize that marked weight loss after bariatric surgery recovers skeletal muscle mitochondrial function.
METHODS: Skeletal muscle mitochondrial function as assessed by high-resolution respirometry was measured in 8 morbidly obese patients (body mass index [BMI], 41.3±4.7 kg/m2; body fat, 48.3%±5.2%) before and 1 year after bariatric surgery (mean weight loss: 35.0±8.6 kg). The results were compared with a lean (BMI 22.8±1.1 kg/m2; body fat, 15.6%±4.7%) and obese (BMI 33.5±4.2 kg/m2; body fat, 34.1%±6.3%) control group.
RESULTS: Before surgery, adenosine diphosphate (ADP)-stimulated (state 3) respiration on glutamate/succinate was decreased compared with lean patients (9.5±2.4 versus 15.6±4.4 O2 flux/mtDNA; P<.05). One year after surgery, mitochondrial function was comparable to that of lean controls (after weight loss, 12.3±5.5; lean, 15.6±4.4 O2 flux/mtDNA). In addition, we observed an increased state 3 respiration on a lipid substrate after weight loss (10.0±3.2 versus 14.0±6.6 O2 flux/mtDNA; P< .05).
CONCLUSIONS: We conclude that impaired skeletal muscle mitochondrial function is a consequence of obesity that recovers after marked weight loss.
• Keywords: Gastric banding; Morbid obesity; Skeletal muscle mitochondrial function; Weight loss
• O2k-Network Lab: NL Maastricht Schrauwen P
Labels: MiParea: Respiration Pathology: Obesity
Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k