Uribe-Alvarez 2016 Abstract MitoFit Science Camp 2016

In Staphylococcus epidermidis, oxygen variations promote differential expression of respiratory enzymes that constitute possible therapeutic targets.

Link:

Uribe-Alvarez C, Chiquete-Felix N, Contreras-Zentella M, Guerrero-Castillo S, Pena A, Uribe-Carvajal S (2016)

Event: MitoFit Science Camp 2016 Kuehtai AT

Staphylcoccus epidermidis does not invade healthy tissues, however, it has been identified as a cause of nosocomial infections due to its ability to form biofilms on polymer surfaces [1]. S. epidermidis can be grown at different oxygen concentrations ([O₂]), including mammalian skin where [O₂] ranges from 3-5% and in anaerobic altered tissues [2,3].

Biofilm formation of S. epidermidis and its respiratory chain components grown in aerobic, microaerobic and anaerobic conditions were evaluated by in-gel activities, enzymatic activities, spectrophotometry and oxymetry. Varying [O₂] modified both biofilm formation and the components in the respiratory chain: At high [O₂], little tendency to form biofilms was observed. S. epidermidis expressed glycerol-3-phosphate, pyruvate, ethanol and succinate dehydrogenases; and cyt bo and aa3. Under micro-aerobiosis, biofilm formation increased slightly; pyruvate, ethanol, glycerol-3-phosphate and succinate dehydrogenase decreased; aa3 cyt was not detected; Under anaerobiosis high biofilm-formation and low ethanol and pyruvate dehydrogenase activities were found; anaerobic nitrate dehydrogenase activity was detected. Aerobic-grown cells with cyanide increased biofilm formation. Anaerobic-grown cells with methylamine decreased biofilm formation.

Thus, either a decrease in [O₂] or the inhibition of the aerobic chain led S. epidermidis to associate into biofilms. In contrast, high [O₂] or inhibition of the anaerobic nitrate reductase prevented biofilm formation suggesting that the enzymes expressed at low to null [O₂] are therapeutic targets against biofilm formation by S. epidermidis.

• O2k-Network Lab: MX Mexico City Uribe-Carvajal S

Labels:

Organism: Eubacteria 

Regulation: Oxygen kinetics 

HRR: Oxygraph-2k  Event: B2  MitoFit Science Camp 2016 

Affiliations

1-Dept Mol Genet, IFC, UNAM, Mexico; 2-Dept Cell Developm Biol, IFC, UNAM, Mexico, 3-Nijmegen Center Mitochondrial Disorders, Radboud Univ Med Center, The Netherlands. - [email protected]

References

1. Otto M (2009) Staphylococcus epidermidis-the 'accidental' pathogen. Nat Rev Microbiol 7:555-67.
2. Peyssonnaux C, Boutin AT, Zinkernagel AS, Datta V, Nizet V, Johnson RS (2008) Critical role of HIF-1alpha in keratinocyte defense against bacterial infection. J Invest Dermatol 128:1964-8.
3. Wiese M, Gerlach RG, Popp I, Matuszak J, Mahapatro M, Castiglione K, Chakravortty D, Willam C, Hensel M, Bogdan C, Jantsch J (2012) Hypoxia-mediated impairment of the mitochondrial respiratory chain inhibits the bactericidal activity of macrophages. Infect Immun 80:1455-66.