Template:SUIT text D110

SUIT-033 O2 mt D110 is used as a control protocol for SUIT-033 NADH mt D081, allowing for cytochrome c test and the evaluation of mitochondrial respiration in OXPHOS in the N-pathway. In the absence of ATPases in the sample, LEAK state can also be evaluated. In order to measure LEAK in the presence of ATPases, this protocol should be performed in parallel to SUIT-032 O2 mt D109.

After the addition of mitochondria in the absence of fuel substrates and ADP, Ren, respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured. The titration of a small concentration of ADP stimulates the depletion of the endogenous substrates, leading to a decrease in mitochondria respiration.

The addition of the complex III inhibitor myxothiazol allows the measurement of Rox.