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Template:SUIT text D035

From Bioblast

SUIT-021 O2 mt D035 is design to assess the additivity between N-pathway and S- pathway in the Q-junction and to investigate the N- and NS-pathway control state in mitochondrial preparations . Oligomycin (Omy) is used to induce a LEAK state of respiration through inhibition of the ATP synthase. Higher concentration of Omy can decreases the ET state initiated by an uncoupler, therefore the required concentration of Omy has to be determined. Uncoupler increases the respiration and induces the ET state. Antimycin A (Ama), which is an inhibitor of Complex III), blocks the respiration. Originially this is a protocol for simultaneous determination of O2 flux and mitochondrial membrane potential on isolated mitochondria and tissue homogenate. It can be used as a control experiment for SUIT-021 Fluo mt D036 without using the fluorescence dye in order to evaluate its effect on the O2 flux as a control.