Template:Chemical SUIT-034 NADH mt D082
From Bioblast
| Step | Chemical(s) and link(s) | Comments |
|---|---|---|
| 1D.1 | ADP (D) | 40 mM stock solution. |
| 2PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
| 3D2.5 | ADP (D) | 500 mM stock solution. |
| 4U | SF6847 | We do not recommend using any other uncoupler, e.g. Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to the chemical background effect on fluorescence. |
| 5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
| 6Myx | Myxothiazol | We do not recommend using any other inhibitor of complex III, e.g. Antimycin A (Ama), due to the chemical background effect on fluorescence. |
| 7Reox | Reoxygenation can be performed by opening the chamber, see: Open chamber. |
