Submitochondrial particles (smtp) consist of membrane fragments which retain most of the enzymatic machinery required in electron transfer and oxidative phosphorylation. Such membrane fragments are continuous closed vesicles formed by resealing of mt-membrane fragments after disruption of the mitochondrial structure. smtp are used to isolate the inner-membrane-bound ET pathway (mETS) from the upstream modules of the Electron transfer pathway (ETS) which are located in the mt-matrix and outer mt-membrane (transporters). smtp are obtained by treatment of mitochondria with membrane-dispersing agents such as digitonin at high concentration or by sonic irradiation.
Reference: Droese 2009 Biochim Biophys Acta
MitoPedia topics: Sample preparation
- It is suggested to replace other frequently used abbreviations for submitochondrial particle (such as SMP) by smtp, with reference to mt as the common abbreviation for mitochondria.