Search results

• D-number
  ...l membrane potential|Fluo]], [[MgG]]). Since the use of different kinds of sample or application may result in slightly different steps, each protocol receiv ...ises different related protocols, each specifically designed for different sample preparations:
  2 KB (251 words) - 14:32, 5 August 2019
• Permeabilized tissue
  '''Addition of sample to the respirometer chamber:''' For permeabilized tissue, remove the Stoppe |mitopedia topic=Sample preparation
  951 bytes (115 words) - 17:08, 26 May 2020
• Markova 2016 Abstract MitoFit Science Camp 2016
  ...reparation from experimental pigs with minimal mitochondrial damage in the sample and sufficient penetration of the substrates utilized during respirometry. ...was inserted into a Falcon tube with ice-cold BIOPS. Approximately 2mg of sample tissue were cut off on ice from the left ventricle and were processed by tw
  3 KB (450 words) - 15:25, 23 January 2019
• Sample
  ...ation is improved, if the symbol ''N'' is used for the number of [[primary sample]]s taken from a study group, and the symbol ''n'' is used for the number of |mitopedia topic=Sample preparation
  6 KB (956 words) - 16:48, 21 February 2024
• O2k-Virtual Support self-study material
  '''Sample types,mitochondrial preparations and sample addition:''' :::* [[Sample]]
  5 KB (754 words) - 12:50, 7 November 2023
• Select plots - DatLab
  | ''c'') In addition, on correction for instrumental O₂ background flux; edit in [Flu | ''d'') In addition, on information about sample concentration; edit [F3]: Million cells.
  5 KB (769 words) - 15:28, 11 April 2018
• Schinagl 2016 PLoS One
  ...y. The degree of inhibition strongly depended on the sequence of inhibitor addition. This suggested, as postulated for plants, that the alternative terminal ox
  3 KB (386 words) - 15:46, 14 October 2023
• SUIT-026 O2 mt D063
  ...dyes may inhibit components of the ET system) and quality control for the sample for [[SUIT-026 AmR mt D064]]. ...a_analysis|mt outer membrane integrity]] provides a quality control of the sample, which cannot be performed in the protocol with Amplex UltraRed.
  2 KB (254 words) - 00:01, 13 April 2021
• Mark statistics - DatLab
  ...f the previous layout was displaying the O2 slope negative, the calculated sample-specific flux will not be automatically background corrected. To obtain the ...tion, exported data include information contained in the [[Sample_-_DatLab|Sample window]], information contained in the [[O2_calibration_-_DatLab#Air_calibr
  6 KB (916 words) - 20:54, 11 June 2023
• Razak 2018 RUMeS
  ...P) uncoupler. Residual oxygen consumption (ROX) was obtained following the addition of complex I inhibitor (rotenone) and complex III inhibitor (antimycin A). ...aningful information regarding the mitochondrial bioenergetics states of a sample.
  2 KB (322 words) - 11:47, 8 April 2019
• Flux analysis - DatLab
  == Smoothing, noise, and sample stability == ...hibitor. Flux may increase over time due to (''1'') a slow response to the addition of ADP or a fuel substrate, or (''2'') an undershoot observed in rare cases
  4 KB (640 words) - 11:46, 25 March 2022
• Permer 2017 Bachelor Thesis
  ...f the fact in which laboratory the measurement has been performed. Such as sample has to be stable for a certain amount of time and has to deliver the same r ...titration protocol, the use of intact HEK293T cells for a stable reference sample appears to be a promising possibility.
  3 KB (456 words) - 16:46, 23 January 2019
• Pacheco 2008 Master Thesis
  muscle from the same biopsy sample. The purpose of this study is to test the functional capacity of mitochondr ...fresh muscle tissue. The significant increase in state 3 respiration upon addition of cytochrome c indicates that cryopreservation causes results in cytochrom
  2 KB (285 words) - 09:40, 8 November 2016
• SUIT-026 O2 ce-pce D088
  ...dyes may inhibit components of the ET system) and quality control for the sample for [[SUIT-026 AmR ce-pce D088]]. ...a_analysis|mt outer membrane integrity]] provides a quality control of the sample, which cannot be performed in the protocol with Amplex UltraRed.
  2 KB (296 words) - 12:04, 5 September 2022
• SUIT-034 NADH mt D082
  ...dized [[NAD]] can be obtained due to low ADP (0.1 μM) titration before the addition of fuel substrates in contrast to [[SUIT-006 NADH mt D084]]. :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state.
  3 KB (454 words) - 16:56, 21 December 2023
• SUIT-033 NADH mt D081
  ...dized [[NAD]] can be obtained due to low ADP (0.1 μM) titration before the addition of fuel substrates in contrast to [[SUIT-032 NADH mt D078]] or [[SUIT-006 N :::- When ATPases are present in the sample, it is not possible to reach the [[LEAK]] state.
  3 KB (462 words) - 17:44, 4 January 2024
• Ost 2018 Curr Opin Clin Nutr Metab Care
  ...s mitochondrial respiratory function in tissue biopsies or blood cells. In addition, it summarizes the recent literature related to this topic. ...tandard operating procedures are required regarding instrumental settings, sample collection and preparation, protocol design and respirometric data analysis
  2 KB (311 words) - 14:40, 5 July 2023
• SUIT-018 AmR mt D040
  ...is to reduce the oxygen concentration in the O2k-Chamber before the sample addition. To decrease or increase the oxygen concentration in the O2k-Chamber, see [ ...ary reoxygenations (high sample concentration) or very long protocols (low sample concentration causing slower oxygen consumption in the O2k-Chamber and thus
  4 KB (625 words) - 12:09, 7 March 2024
• Rhodamine 123
  ...should be tested for their effect in a background run without a biological sample, and the necessary corrections are applied. ...The fluorescence signal was calibrated by Rhodamine 123 before adding the sample in a step-wise titration (max. concentration 1 µM). The optimum concentrat
  5 KB (698 words) - 15:50, 16 March 2021
• Krajcova 2018 MiPschool Tromso E2
  ...ue biopsy (isolated mitochondria) or lengthy and time-consuming process of sample preparation (permeabilized muscle fibers). In this study we aimed to develo ...CP and 4 µM antimycin. The technique requires only 20 mg of myocardium and sample preparation takes less than 20 min.
  3 KB (460 words) - 23:10, 19 October 2018

View (previous 20 | next 20) (20 | 50 | 100 | 250 | 500)