Palmitoylcarnitine

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Palmitoylcarnitine

Description

Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.

Abbreviation: Pal

Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.


Application in HRR

Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl, C23H45N04HCl); Sigma P 4509, 100 mg, store at -20 °C; FW = 436.1.
Preparation of 10 mM stock solution (dissolved in H2O):
  1. Weigh 8.72 mg of palmitoylcarnitine.
  2. Dissolve in 2 mL H2O.
  3. Divide into 0.2 mL portions.
  4. Store frozen at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 8 µL using a 25 µL syringe (2 mL O2k-chamber).
  • Final concentration: 0.04 mM.



MitoPedia topics: Substrate and metabolite