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Oemer 2016a Abstract Mito Xmas Meeting Innsbruck

From Bioblast
Mass spectrometric characterisation of cardiolipins reveals detailed insights into the structural diversity of mitochondrial phospholipids.

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Oemer G, Watschinger K, Werner ER, Zschocke J, Keller MA (2016)

Event: Mito Xmas Meeting 2016 Innsbruck AT

The mitochondrial glycerophospholipid class of cardiolipins possess a unique structure, as they can carry up to four acyl side chains and are able to trap protons by phosphate-glycerol bicyclic resonance structure. They are almost exclusively found in the inner mitochondrial membranes where they are functionally involved in maintaining oxidative phosphorylation by stabilising transmembrane proteins, buffering fluctuations of the proton gradient, protecting mtDNA, and mediating oxidative damage - related apoptotic signalling events. Interestingly, a highly regulated tissue-specific fatty acyl side chain composition of cardiolipins is required to establish maximum functional efficiency. The exact patterns of acyl substitutions are established in a post-biosynthetic remodelling process requiring the Barth Syndrome linked protein tafazzin.

We have recently developed a reversed-phase high performance liquid chromatography – tandem mass spectrometric methodology, enabling the identification and absolute quantification of up to 140 different cardiolipins within biological samples such as bacteria, unicellular eukaryotes and tissues of multicellular organisms. In contrast to most previous approaches, that only focus on few targeted cardiolipin subspecies, we are able to analyse a broad spectrum of cardiolipins, including their respective monolyso- and oxidised/peroxidised counterparts, resulting in a complete cardiolipidome. Furthermore, by mathematical modelling of MS2 spectra generated by data-dependent fragmentation, it was possible to reveal the specific fatty acyl compositions of individual cardiolipin species, and to distinguish specific intramolecular side chain and double bond distributions.

Thus, the here presented cardiolipidomics approach opens up the possibility to comprehensively characterise this mitochondrial signature lipid and to study its functional roles in a broad range of samples.


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Event: Poster 


Affiliations

Γ–mer G(1), Watschinger K(2), Werner ER(2), Zschocke J(1), Keller MA(1)
  1. Div Human Genetics, Medical Univ Innsbruck, Austria
  2. Div Biological Chemistry, Medical Univ Innsbruck, Austria