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Moriyama 2016 J Surg Res

From Bioblast
Publications in the MiPMap
Moriyama T, Kanmura Y, Lindahl SG (2016) Atrial natriuretic peptide attenuation of renal ischemia-reperfusion injury after major surgery. J Surg Res 201:213-8.

» PMID: 26850205

Moriyama T, Kanmura Y, Lindahl SG (2016) J Surg Res

Abstract: Ischemia-reperfusion (I/R) injury is one of the most important pathologic processes causing acute kidney injury. Human atrial natriuretic peptide (hANP) has various effects, including renal protection. The purpose of the present work was to study the effects of intrarenal angiotensin II (Ang II) and investigate the potential of hANP to prevent kidney injury.

Male Sprague-Dawley rats were divided into three groups as follows: (1) sham; (2) I/R (30 min of bilateral renal ischemia followed by 6 h reperfusion); and (3) I/R + hANP (I/R injury + continuous intravenous infusion of hANP at 0.025 μg/kg/min). After 6 h of reperfusion, both renal and plasma Ang II concentrations were measured. Urinary angiotensinogen and neutrophil gelatinase-associated lipocalin were measured before ischemia and 2, 4, and 6 h after reperfusion. To evaluate the renal-protective effects of hANP, serum creatinine was determined 6 and 24 h after reperfusion. In addition, mitochondrial oxygen consumption in kidney cortex was measured in the presence of Ang II and hANP.

Renal Ang II concentrations were 24.5 ± 3.9 and 14.2 ± 3.4 pg/mg renal weight in the I/R and I/R + hANP groups, respectively. Urinary angiotensinogen and neutrophil gelatinase-associated lipocalin excretions were elevated after I/R injury. Treatment with hANP significantly attenuated this effect after 4 and 6 h. Oxygen consumption in renal mitochondria increased with the addition of Ang II, which was also attenuated by hANP.

Production of intrarenal Ang II was attenuated by hANP, indicating a potential to diminish renal I/R injury.

Copyright © 2016 Elsevier Inc. All rights reserved. Keywords: Acute kidney injury, Atrial natriuretic peptide, Intrarenal angiotensin II, Ischemia–reperfusion, Mitochondria, Oxygen consumption Bioblast editor: Kandolf G


Labels: MiParea: Respiration, Pharmacology;toxicology 

Stress:Ischemia-reperfusion  Organism: Rat  Tissue;cell: Kidney  Preparation: Isolated mitochondria 



HRR: Oxygraph-2k