MiPNet22.04 IOC120 Barcelona ES

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Oroboros Instruments

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MiPNet22.04 IOC120 Barcelona ES

Publications in the MiPMap
O2k-Network

Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) IOC120. »ES Barcelona Zorzano A

» Bioblast pdf

Oroboros (2017-03-20) Mitochondr Physiol Network

Abstract:
O2k-Workshops.png

O2k-Workshop on high-resolution respirometry - satellite to MitoEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.


O2k-Network Lab: AT_Innsbruck_Oroboros, ES Barcelona Zorzano A


General information

Programme

Monday 20th Topic Weblink
08:45 Registration
09:00-09:15 Welcome by Prof. Antonio Zorzano.
09:15-09:30 Introduction of participants and their research interests. IOC120
09:30-10:00 Get started with the O2k: Overview with video clips. O2k-Manual
10:00-11:30 Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay. The Blue Book - SUIT reference protocol
11:30-12:00 Coffee/tea break
12:00-13:00 Principles of high-resolution respirometry: From switching

on the O2k to the experimental result – oxygen sensor calibration and quality control.

POS-calibration-SOP
13:00-14:00 O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol. O2k-Fluorometry
14:00-15:00 Lunch
15:00-17:30 Demo-Experiment: HRR and O2k-Fluorometry with liver mitochondria – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2. RP1 - RP2
17:30-18:30 Protocol driver for Marks and data analysis.
18:30-20:00 The Bioblast wiki, O2k-Network and feedback discussion.

Venue

Barcelona Science Park
Room 1 Tower D
R+D+I Towers building
C/ Baldiri Reixac 4-8
08028 Barcelona


To get to the workhsop location following routes are possible:
  • Start in Barcelona centrum: easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination
  • Another option is to take a bus(see picture below with bus plan)
  • Start airport: take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min
  • Taxi will take about 20 min and the costs are approximately 20-25 €
  • Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial



Registration

  • Please register here for the O2k-Workshop.
  • Registration deadline: March 13


Lecturers and tutors


List of participants

Participant Institution
Susana Barros
Barros Susana ES_Barcelona_Zorzano A: IRB Barcelona (ES)
Martín Cabezuelo Rubén Universitat Politècnica de València (UPV)
Gama Perez Pau ES Barcelona Garcia-Roves PM : Physiological Science Department, University of Barcelona (ES)
Garten Antje UK_Birmingham_Lavery GG: Institute of Metabolism and Systems Research, University of Birmingham (UK)
Giovarelli Matteo IT_Milan_Clementi_E: Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT)
Gonzalo Hugo ES Lleida Boada J: IRB Lleida (ES)
Granado Serrano Ana Belén ES Lleida Boada J: IRB Barcelona (ES)
Laura Herrero
Herrero Laura Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES)
Javier Iglesias-Gonzalez
Iglesias-Gonzalez Javier UK_Manchester_Galli GL: Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester (UK)
Thomas Ingram
Ingram Thomas University of Nottingham (UK)
Magdalena König
Koenig Magdalena Goethe University, Frankfurt (DE)
Lyakhovich Alexander Research Institute VHIR, Barcelona (ES)
Michal Nemec
Nemec Michal Institute of Experimental Endocrinology, Bratislava (SK)
Nogueras Lara ES Lleida Boada J: IRB Lleida (ES)
Mario Ost
Ost Mario DE Nuthetal Klaus S: Department of Pharmacology, German Institute of Human Nutrition (DE)
Rivera-Ingraham Georgina Université de Montpellier (FR)
Sabate Alba ES Barcelona Zorzano A: IRB Barcelona (ES)
Sánchez Feutrie Manuela ES Barcelona Zorzano A: Department of Biochemistry and Molecular Biology, University of Barceona (ES)
David Sebastián
Sebastián David ES_Barcelona_Zorzano A: IRB Barcelona (ES)
Soler Vázquez Maria Del Carmen Department of Biochemistry and Physiology, University of Barcelona (ES)
Anna Thinnes
Thinnes Anna Goethe University, Frankfurt (DE)
Zagmutt Caroca Sebastián University of Barcelona (ES)


Contact

instruments@oroboros.at


Oroboros Instruments
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
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IOC recommended reading

» IOC recommended reading



MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


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HRR: Oxygraph-2k 

ORO, 2017, IOC, O2k-Network