Jimenez-Aranda 2014 J Pineal Res

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Jimenéz-Aranda A, Fernández-Vázquez G, Serrano MM, Reiter RJ, Agil A (2014) Melatonin improves mitochondrial function in inguinal white adipose tissue of Zücker diabetic fatty rats. J Pineal Res 57:103-9.

» PMID:24867433

Jimenez-Aranda A, Fernandez-Vazquez G, Serrano MM, Reiter RJ, Agil A. (2014) J Pineal Res

Abstract: Mitochondrial dysfunction in adipose tissue may contribute to obesity-related metabolic derangements such as type 2 diabetes mellitus (T2DM). Because mitochondria are a target for melatonin action, the goal of present study was to investigate the effects of melatonin on mitochondrial function in white (WAT) and beige inguinal adipose tissue of Zücker diabetic fatty (ZDF) rats, a model of obesity-related T2DM. In this experimental model melatonin reduces obesity and improves the metabolic profile. At 6 weeks of age, ZDF rats and lean littermates (ZL) were subdivided into two groups, each composed of four rats: control (C-ZDF and C-ZL) and treated with oral melatonin in the drinking water (10 mg/kg/day) for 6 weeks (M-ZDF and M-ZL). After the treatment period, animals were sacrificed, tissues dissected and mitochondrial function assessed in isolated organelles. Melatonin increased the respiratory control ratio (RCR) in mitochondria from white fat of both lean (by 26.5%, p<0.01) and obese (by 34.5%, p<0.01) rats mainly through a reduction of proton leaking component of respiration (state 4) (28% decrease in ZL, p<0.01 and 35% in ZDF, p<0.01). However, melatonin treatment lowered the RCR in beige mitochondria of both lean (by 7%, p<0.05) and obese (by 13%, p<0.05) rats by maintaining high rates of uncoupled respiration. Melatonin also lowered mitochondrial oxidative status by reducing nitrite levels and by increasing superoxide dismutase activity. Moreover, melatonin treatment also caused a profound inhibition of Ca-induced opening of mPTP in isolated mitochondria from both types of fat, white and beige, in both lean and obese rats. These results demonstrate that chronic oral melatonin improves mitochondrial respiration, and reduces the oxidative status and susceptibility to apoptosis in white and beige adipocytes. These melatonin effects help to prevent mitochondrial dysfunction and thereby to improve obesity-related metabolic disorders such as diabetes and dyslipidemia of ZDF rats. This article is protected by copyright. All rights reserved.

Keywords: Melatonin, White adipose tissue, Beige adipose tissue, Mitochondrial function, Zuecker diabetic fatty rats

Labels: MiParea: Respiration, Pharmacology;toxicology  Pathology: Diabetes, Obesity 

Organism: Rat  Tissue;cell: Fat  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS  Pathway: N, S  HRR: Oxygraph-2k