Hecker 2015 Methods Mol Biol

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Hecker M, Sommer N, Mayer K (2015) Assessment of short- and medium-chain fatty acids on mitochondrial function in severe Inflammation. Methods Mol Biol 1265:389-96.

» PMID:25634290

Hecker M, Sommer N, Mayer K (2015) Methods Mol Biol

Abstract: Mitochondrial dysfunction is regarded as one key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The infl uence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under infl ammatory conditions has thus far not been investigated. In the following protocol, we describe the assessment of mitochondrial respiration using high-resolution respirometry under infl ammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-α to mimic infl ammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR.

Keywords: Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-α, HUVEC

O2k-Network Lab: DE Giessen Weissmann N

Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics  Pathology: Sepsis 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Intact cells 

Regulation: Coupling efficiency;uncoupling, Uncoupler, Fatty acid  Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k