Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Debska 2002 Biochim Biophys Acta

From Bioblast
Publications in the MiPMap
Debska G, Kicinska A, Skalska J, Szewczyk A, May R, Elger CE, Kunz WS (2002) Opening of potassium channels modulates mitochondrial function in rat skeletal muscle. Biochim Biophys Acta 1556:97-105.

Β» PMID: 12460666

Debska G, Kicinska A, Skalska J, Szewczyk A, May R, Elger CE, Kunz WS (2002) Biochim Biophys Acta

Abstract: We have investigated the presence of diazoxide- and nicorandil-activated K+ channels in rat skeletal muscle. Activation of potassium transport in the rat skeletal muscle myoblast cell line L6 caused a stimulation of cellular oxygen consumption, implying a mitochondrial effect. Working with isolated rat skeletal muscle mitochondria, both potassium channel openers (KCOs) stimulate respiration, depolarize the mitochondrial inner membrane and lead to oxidation of the mitochondrial NAD-system in a strict potassium-dependent manner. This is a strong indication for KCO-mediated stimulation of potassium transport at the mitochondrial inner membrane. Moreover, the potassium-specific effects of both diazoxide and nicorandil on oxidative phosphorylation in skeletal muscle mitochondria were completely abolished by the antidiabetic sulfonylurea derivative glibenclamide, a well-known inhibitor of ATP-regulated potassium channels (Katp channels). Since both diazoxide and nicorandil facilitated swelling of de-energised mitochondria in KSCN buffer at the same concentrations, our results implicate the presence of a mitochondrial ATP-regulated potassium channel (mitoKatp channel) in rat skeletal muscle which can modulate mitochondrial oxidative phosphorylation. β€’ Keywords: Skeletal muscle, Mitochondrial ATP-regulated potassium channel, Potassium channel opener, Oxidative phosphorylation

β€’ O2k-Network Lab: PL Warsaw Szewczyk A


Labels:


Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 



HRR: Oxygraph-2k