Davis 2023 MitoFit
| Davis MS, Barrett MR, Bayly WM, Bolinger A (2023) Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration. MitoFit Preprints 2023.5. https://doi.org/10.26124/mitofit:2023-0005 |
» MitoFit Preprints 2023.5.
Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration.
Davis Michael S, Barrett Montana R, Bayly Warwick M, Bolinger Amanda (2023) MitoFit Prep
Abstract: Equine skeletal muscle provides a rich source of mitochondria, suitable for both basic and applied studies of cellular energetics. Inclusion of fluorophores and the associated endpoints provides valuable information to complement measurements of mitochondrial oxygen flux, but investigators must be aware of the possible artifacts created by those fluorophores. Our studies demonstrate that Magnesium Green has no significant effect on respiration of isolated mitochondria, whereas tetramethylrhodamine inhibits phosphorylating respiration and increases leak respiration. Amplex UltraRed increases phosphorylating and non-phosphorylating respiration through Complex I. The effect of Amplex UltraRed is a novel finding, and further study is necessary to determine the mechanism underlying this artifact.
• Keywords: horse; Amplex UltraRed; Magnesium Green; tetramethylrhodamine; LEAK respiration
• Bioblast editor: Tindle-Solomon L
• O2k-Network Lab: US OK Stillwater Davis MS
Labels: MiParea: Respiration
Organism: Horse
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS
Pathway: N, S
HRR: Oxygraph-2k
