Crisan 2008 Stem Cells

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Publications in the MiPMap
Crisan M, Casteilla L, Lehr L, Carmona OMC, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP (2008) A reservoir of brown adipocyte progenitors in human skeletal muscle. Stem Cells 26:2425-33.

» PMID: 18617684 Open Access

Crisan M, Casteilla L, Lehr L, Carmona OMC, Paoloni-Giacobino A, Yap S, Sun Bin, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP (2008) Stem Cells

Abstract: Brown adipose tissue uncoupling protein-1 (UCP1) plays a major role in the control of energy balance in rodents. It has long been thought, however, that there is no physiologically relevant UCP1 expression in adult humans. In this study we show, using an original approach consisting of sorting cells from various tissues and differentiating them in an adipogenic medium, that a stationary population of skeletal muscle cells expressing the CD34 surface protein can differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression and uncoupled respiration. These cells can be expanded in culture, and their UCP1 mRNA expression is strongly increased by cell-permeating cAMP derivatives and a peroxisome-proliferator-activated receptor-gamma (PPARgamma) agonist. Furthermore, UCP1 mRNA was detected in the skeletal muscle of adult humans, and its expression was increased in vivo by PPARgamma agonist treatment. All the studies concerning UCP1 expression in adult humans have until now been focused on the white adipose tissue. Here we show for the first time the existence in human skeletal muscle and the prospective isolation of progenitor cells with a high potential for UCP1 expression. The discovery of this reservoir generates a new hope of treating obesity by acting on energy dissipation.

Keywords: Brown adipocytes, Human muscle

O2k-Network Lab: FR Toulouse Casteilla L, NL Maastricht Schrauwen P, ES Barcelona Gomis R


Labels: MiParea: Respiration, Genetic knockout;overexpression, mt-Medicine 


Organism: Human, Mouse  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Intact cells  Enzyme: Uncoupling protein  Regulation: Uncoupler  Coupling state: OXPHOS 

HRR: Oxygraph-2k